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Glucose Transport

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Karin Lindkvist-Petersson

    1. Department of Experimental Medical Science, Lund University, Lund, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. Jesper S Hansen

    1. Department of Experimental Medical Science, Lund University, Lund, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1713)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Expression and Purification of Rat Glucose Transporter 1 in Pichia pastoris

    • Raminta Venskutonytė, Karin Elbing, Karin Lindkvist-Petersson
    Pages 1-13

  3. Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3

    • Dong Deng, Nieng Yan
    Pages 15-29

  4. Screening and Scale-Up of GLUT Transporter Constructs Suitable for Biochemical and Structural Studies

    • Grégory Verdon, Hae Joo Kang, David Drew
    Pages 31-43

  5. GLUT Characterization Using Frog Xenopus laevis Oocytes

    • Wentong Long, Debbie O’Neill, Chris I. Cheeseman
    Pages 45-55

  6. Glucose Uptake in Heterologous Expression Systems

    • Eunice E. Lee, Richard C. Wang
    Pages 57-67

  7. Evaluating the Efficacy of GLUT Inhibitors Using a Seahorse Extracellular Flux Analyzer

    • Changyong Wei, Monique Heitmeier, Paul W. Hruz, Mala Shanmugam
    Pages 69-75

  8. Glucose Transport Activity Measured in Giant Vesicles

    • Jesper S. Hansen, Karin Lindkvist-Petersson
    Pages 77-91

  9. Design, Synthesis, and Evaluation of GLUT Inhibitors

    • Carlotta Granchi, Tiziano Tuccinardi, Filippo Minutolo
    Pages 93-108

  10. Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level

    • Niek Welkenhuysen, Caroline B. Adiels, Mattias Goksör, Stefan Hohmann
    Pages 109-121

  11. A Growth-Based Screening System for Hexose Transporters in Yeast

    • Eckhard Boles, Mislav Oreb
    Pages 123-135

  12. Identification of Insulin-Activated Rab Proteins in Adipose Cells Using Bio-ATB-GTP Photolabeling Technique

    • Francoise Koumanov, Geoffrey D. Holman
    Pages 137-150

  13. Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking

    • Sebastian Wasserstrom, Björn Morén, Karin G. Stenkula
    Pages 151-159

  14. Translocation and Redistribution of GLUT4 Using a Dual-Labeled Reporter Assay

    • Robert M. Jackson, Ann Louise Olson
    Pages 161-173

  15. GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence

    • Javier R. Jaldin-Fincati, Philip J. Bilan, Amira Klip
    Pages 175-192

  16. Glucose Transport: Methods for Interrogating GLUT4 Trafficking in Adipocytes

    • Dougall M. Norris, Tom A. Geddes, David E. James, Daniel J. Fazakerley, James G. Burchfield
    Pages 193-215

  17. Proximity Ligation Assay to Study the GLUT4 Membrane Trafficking Machinery

    • Dimitrios Kioumourtzoglou, Gwyn W. Gould, Nia J. Bryant
    Pages 217-227

  18. Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay

    • Zahra Maria, Véronique A. Lacombe
    Pages 229-240

  19. Tracking GLUT2 Translocation by Live-Cell Imaging

    • Sabina Tsytkin-Kirschenzweig, Merav Cohen, Yaakov Nahmias
    Pages 241-254

  20. GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis

    • Gwenaël Labouèbe, Bernard Thorens, Christophe Lamy
    Pages 255-267
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Keywords

About this book

This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologous expression systems, crystal structure determination, glucose transport activity assays in cellular and synthetic model systems, investigation of the GLUT4 membrane trafficking machinery by novel labeling strategies combined with advanced fluorescence microscopy methods, and tracking GLUT2 translocation by live cell imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Glucose Transport: Methods and Protocols is a valuable resource that helps researchers with determining specific roles of different GLUTs in various organisms.


Editors and Affiliations

  • Department of Experimental Medical Science, Lund University, Lund, Sweden

    Karin Lindkvist-Petersson, Jesper S Hansen

Bibliographic Information

  • Book Title: Glucose Transport

  • Book Subtitle: Methods and Protocols

  • Editors: Karin Lindkvist-Petersson, Jesper S Hansen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7507-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7506-8Published: 08 December 2017

  • Softcover ISBN: 978-1-4939-8506-7Published: 22 June 2018

  • eBook ISBN: 978-1-4939-7507-5Published: 07 December 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 274

  • Number of Illustrations: 34 b/w illustrations, 34 illustrations in colour

  • Topics: Cell Biology

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