Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1703)
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Table of contents (22 protocols)
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Front Matter
About this book
This volume looks at the evolution of the topo field over the last year and discusses how DNA topoisomerases are directly and indirectly involved in numerous cellular processes. The chapters in this book cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental protocols related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; protocols for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmid-mediated quinolone resistance in bacteria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, DNA Topoisomerases: Methods and Protoc
ols is intended for specialists and non-specialists with an overview of selected hot topics in the field, and the latest protocols to study DNA topology, topoisomerases functions and inhibition by various compounds.
Editors and Affiliations
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Département de microbiologie, infectiologie et immunologie, Université de Montréal, Montréal, Canada
Marc Drolet
Bibliographic Information
Book Title: DNA Topoisomerases
Book Subtitle: Methods and Protocols
Editors: Marc Drolet
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7459-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2018
Hardcover ISBN: 978-1-4939-7458-0Published: 25 November 2017
Softcover ISBN: 978-1-4939-8492-3Published: 30 August 2018
eBook ISBN: 978-1-4939-7459-7Published: 23 November 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 328
Number of Illustrations: 46 b/w illustrations, 21 illustrations in colour
Topics: Human Genetics