Overview
- Includes cutting-edge methods and protocols for the study of cellular quiescence
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1686)
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Table of contents (20 protocols)
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Front Matter
Keywords
About this book
This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology.Editors and Affiliations
Bibliographic Information
Book Title: Cellular Quiescence
Book Subtitle: Methods and Protocols
Editors: H. Daniel Lacorazza
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7371-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018
Hardcover ISBN: 978-1-4939-7370-5Published: 18 October 2017
Softcover ISBN: 978-1-4939-8465-7Published: 11 September 2018
eBook ISBN: 978-1-4939-7371-2Published: 13 October 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 303
Number of Illustrations: 17 b/w illustrations, 43 illustrations in colour
Topics: Cell Biology