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Potassium Channels

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Show-Ling Shyng

    1. Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, USA

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  2. Francis I. Valiyaveetil

    1. Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Matt Whorton

    1. The Vollum Institute, Oregon Health and Science University, Portland, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1684)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Manipulating Potassium Channel Expression and Function in Hippocampal Neurons by In Utero Electroporation

    • Kang Wang
    Pages 1-5

  3. Studying KCNQ1 Mutation and Drug Response in Type 1 Long QT Syndrome Using Patient-Specific Induced Pluripotent Stem Cell-Derived Cardiomyocytes

    • Heming Wei, Jianjun Wu, Zhenfeng Liu
    Pages 7-28

  4. Monitoring Changes in the Abundance of Endogenously Expressed ATP-Sensitive Potassium (KATP) Channels in the Plasma Membrane Using Surface Biotinylation

    • Jing-Syuna Ruan, Pei-Chun Chen
    Pages 29-36

  5. Nonsense-Mediated mRNA Decay of hERG Mutations in Long QT Syndrome

    • Qiuming Gong, Zhengfeng Zhou
    Pages 37-49

  6. Probing Subunits Interactions in KATP Channels Using Photo-Crosslinking via Genetically Encoded p-Azido-l-phenylalanine

    • Prasanna Devaraneni, Emily A. Rex, Show-Ling Shyng
    Pages 51-61

  7. Hyper-SUMOylation of K+ Channels in Sudden Unexplained Death in Epilepsy: Isolation and Primary Culture of Dissociated Hippocampal Neurons from Newborn Mice for Subcellular Localization

    • Xu Chen, Shanshan Zhang, Jifang Huang, Wanying Dong, Hui Xiao, Huanjie Shao et al.
    Pages 63-71

  8. Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function

    • Nicholas C. Vierra, Matthew T. Dickerson, Louis H. Philipson, David A. Jacobson
    Pages 73-84

  9. Methods for Characterizing Disease-Associated ATP-Sensitive Potassium Channel Mutations

    • Balamurugan Kandasamy, Show-Ling Shyng
    Pages 85-104

  10. Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters

    • C. David Weaver
    Pages 105-114

  11. Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions

    • Yuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa, Ichio Shimada
    Pages 115-128

  12. Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome Membranes

    • Josefina del Mármol, Robert A. Rietmeijer, Stephen G. Brohawn
    Pages 129-150

  13. Migration of PIP2 on KCNQ2 Surface Revealed by Molecular Dynamics Simulations

    • Qiansen Zhang, Huaiyu Yang
    Pages 151-161

  14. Studying Structural Dynamics of Potassium Channels by Single-Molecule FRET

    • Shizhen Wang, Joshua B. Brettmann, Colin G. Nichols
    Pages 163-180

  15. Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters

    • Kimberly Matulef, Francis I. Valiyaveetil
    Pages 181-191

  16. Combinatorial Assembly of Lumitoxins

    • David Nedrud, Daniel Schmidt
    Pages 193-209

  17. Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay

    • Michael J. Litt, Roger D. Cone, Masoud Ghamari-Langroudi
    Pages 211-222

  18. Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies

    • David J. Posson, Radda Rusinova, Olaf S. Andersen, Crina M. Nimigean
    Pages 223-235

  19. In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish

    • Jui-Yi Hsieh, Diane M. Papazian
    Pages 237-252

  20. Site-Directed Unnatural Amino Acid Mutagenesis to Investigate Potassium Channel Pharmacology in Xenopus laevis Oocytes

    • Robin Y. Kim, Harley T. Kurata
    Pages 253-263
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Keywords

About this book

This volume provides readers with the latest techniques and approaches used to study K+ channels. There are a wide range of topics covered in the book, such as channel structure; K+ channels and their roles in physiology and disease; single-molecule FRET; proteins expression levels in vivo; real-time correlation of channel activity; gating; pharmacology; physiological functions; and channelopathies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Potassium Channels: Methods and Protocols is a valuable resource for all researchers who are interested in studying potassium channels.

Editors and Affiliations

  • Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, USA

    Show-Ling Shyng, Francis I. Valiyaveetil

  • The Vollum Institute, Oregon Health and Science University, Portland, USA

    Matt Whorton

Bibliographic Information

  • Book Title: Potassium Channels

  • Book Subtitle: Methods and Protocols

  • Editors: Show-Ling Shyng, Francis I. Valiyaveetil, Matt Whorton

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7362-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7361-3Published: 24 October 2017

  • Softcover ISBN: 978-1-4939-8462-6Published: 23 August 2018

  • eBook ISBN: 978-1-4939-7362-0Published: 20 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 345

  • Number of Illustrations: 31 b/w illustrations, 53 illustrations in colour

  • Topics: Biochemistry, general

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