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  • © 2017

NMDA Receptors

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1677)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
  2. NMDA Receptors in the Central Nervous System

    • Kasper B. Hansen, Feng Yi, Riley E. Perszyk, Frank S. Menniti, Stephen F. Traynelis
    Pages 1-80
  3. NMDAR Subunits Expression and Mutations

    1. Front Matter

      Pages 81-81
    2. Genetic and Functional Analysis of GRIN2A in Tumor Samples

      • Todd D. Prickett, Jared J. Gartner, Yardena Samuels
      Pages 93-116
    3. Detection of NMDARs Antibodies in Encephalitis

      • Matteo Gastaldi, Patrick Waters, Angela Vincent
      Pages 117-126
  4. Studying Structure–Function Relations in NMDARs

    1. Front Matter

      Pages 127-127
    2. Transfection in Primary Cultured Neuronal Cells

      • Katie F. M. Marwick, Giles E. Hardingham
      Pages 137-144
    3. Selective Cell-Surface Expression of Triheteromeric NMDA Receptors

      • Feng Yi, Stephen F. Traynelis, Kasper B. Hansen
      Pages 145-162
    4. GluN2B Subunit Labeling with Fluorescent Probes and High-Resolution Live Imaging

      • Cécile Perrio, Olivier Nicole, Alain Buisson
      Pages 171-183
  5. Analysis of NMDAR Function/Activity

    1. Front Matter

      Pages 199-199
    2. Gene Targeted Mice with Conditional Knock-In (-Out) of NMDAR Mutations

      • Rolf Sprengel, Ahmed Eltokhi, Frank N. Single
      Pages 201-230
    3. Electrophysiological Investigation of NMDA Current Properties in Brain Slices

      • Alexandre Pons-Bennaceur, Natalia Lozovaya
      Pages 231-239
    4. GluNs Detection and Functions in Microglial Cells

      • Stéphane Peineau, Vincent Degos, Catherine Verney, Pierre Gressens
      Pages 253-263
    5. NMDA Receptor Activity in Circulating Red Blood Cells: Methods of Detection

      • Asya Makhro, Lars Kaestner, Anna Bogdanova
      Pages 265-282
    6. NMDA Receptors as Voltage Sensors

      • Roustem Khazipov
      Pages 283-290

About this book

This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing. The volume includes a detailed introductory chapter describing the field and is divided into three parts. Topics covered in the volume include: quantification of NMDAR subunit genes expression by qRT-PCR; detection of NMDARs antibodies in encephalitis; recombinant channels in host cells using a fast agonist application system; GluNs detection and functions in microglial cells; and NMDARs as voltage sensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, NMDA Receptors: Methods and Protocols is a valuable resource that discusses the diversity of the currently-used methods, the importance of NMDARs and their complexity, and the progress that has already been made. This book will be of interest to scientists, clinicians, and industry professionals working in this field.

Editors and Affiliations

  • Mediterranean Institute of Neurobiology (INMED), Aix-Marseille University, INSERM U901, Marseille, France

    Nail Burnashev, Pierre Szepetowski

Bibliographic Information

  • Book Title: NMDA Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Nail Burnashev, Pierre Szepetowski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7321-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7320-0Published: 07 October 2017

  • Softcover ISBN: 978-1-4939-8452-7Published: 14 August 2018

  • eBook ISBN: 978-1-4939-7321-7Published: 06 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 314

  • Number of Illustrations: 31 b/w illustrations, 46 illustrations in colour

  • Topics: Neurosciences

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access