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  • © 2018

Plant Chromatin Dynamics

Methods and Protocols

  • Includes cutting-edge methods protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1675)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xiv
  2. Profiling of Chromatin States

    1. Front Matter

      Pages 1-1
    2. Profiling Developmentally and Environmentally Controlled Chromatin Reprogramming

      • Clara Bourbousse, Moussa Benhamed, Fredy Barneche
      Pages 3-30
    3. Profiling DNA Methylation Using Bisulfite Sequencing (BS-Seq)

      • Yun-Ru Chen, Sheng Yu, Silin Zhong
      Pages 31-43
    4. Bisulfite Sequencing Using Small DNA Amounts

      • Susanne Edelmann, Stefan Scholten
      Pages 45-60
    5. A Rapid and Efficient ChIP Protocol to Profile Chromatin Binding Proteins and Epigenetic Modifications in Arabidopsis

      • Bénédicte Desvoyes, Zaida Vergara, Joana Sequeira-Mendes, Sofia Madeira, Crisanto Gutierrez
      Pages 71-82
    6. Sequential ChIP Protocol for Profiling Bivalent Epigenetic Modifications (ReChIP)

      • Bénédicte Desvoyes, Joana Sequeira-Mendes, Zaida Vergara, Sofia Madeira, Crisanto Gutierrez
      Pages 83-97
    7. A Method to Identify Nucleolus-Associated Chromatin Domains (NADs)

      • Marie-Christine Carpentier, Ariadna Picart-Picolo, Frédéric Pontvianne
      Pages 99-109
    8. Cell Type-Specific Profiling of Chromatin Modifications and Associated Proteins

      • Ana Karina Morao, Erwann Caillieux, Vincent Colot, François Roudier
      Pages 111-130
    9. Profiling Nucleosome Occupancy by MNase-seq: Experimental Protocol and Computational Analysis

      • Alice Pajoro, Jose M. Muiño, Gerco C. Angenent, Kerstin Kaufmann
      Pages 167-181
    10. Identification of Open Chromatin Regions in Plant Genomes Using ATAC-Seq

      • Marko Bajic, Kelsey A. Maher, Roger B. Deal
      Pages 183-201
  3. Chromatin Dynamics and Gene Regulation

    1. Front Matter

      Pages 203-203
    2. 3C in Maize and Arabidopsis

      • Blaise Weber, Suraj Jamge, Maike Stam
      Pages 247-270
    3. De Novo Identification of sRNA Loci and Non-coding RNAs by High-Throughput Sequencing

      • Alice Lunardon, Cristian Forestan, Silvia Farinati, Serena Varotto
      Pages 297-314

About this book

This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into three sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Chromatin Dynamics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Molecular Biology, Wageningen University & Research, Wageningen, The Netherlands

    Marian Bemer

  • Department of Plant and Microbial Biology, Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland

    Célia Baroux

Bibliographic Information

  • Book Title: Plant Chromatin Dynamics

  • Book Subtitle: Methods and Protocols

  • Editors: Marian Bemer, Célia Baroux

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7318-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7317-0Published: 20 October 2017

  • Softcover ISBN: 978-1-4939-8451-0Published: 23 August 2018

  • eBook ISBN: 978-1-4939-7318-7Published: 20 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 659

  • Number of Illustrations: 24 b/w illustrations, 66 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access