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Maize

Methods and Protocols

  • Book
  • © 2018

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1676)

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Table of contents (13 protocols)

  1. Cell, Tissue, and Organ Culture and Maize Transformation

  2. Gene Silencing and Generation of Mutant Populations

  3. Plant Gene Expression

  4. Plant Metabolic Networks

Keywords

About this book

This volume discusses techniques used for the molecular characterization of maize. This book is divided into 4 parts: cell, tissue, and organ culture and maize transformation; gene silencing and generation of mutant populations; plant gene expression; and plant metabolic networks. The chapters cover a range of topics, such as growing and propagating maize in the laboratory, greenhouse and field studies, screening mutagenic population, characterizing the genome, describing protein and metabolic regulatory networks, and generating transgenic plants for gene knock-out and over expression purposes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Maize: Methods and Protocols is a valuable resource for everyone who is interested in maize research.

Editors and Affiliations

  • Department of Agronomy and Horticulture, University of Nebraska – Lincoln, Lincoln, USA

    L. Mark Lagrimini

Bibliographic Information

  • Book Title: Maize

  • Book Subtitle: Methods and Protocols

  • Editors: L. Mark Lagrimini

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7315-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7314-9Published: 07 October 2017

  • Softcover ISBN: 978-1-4939-8450-3Published: 14 August 2018

  • eBook ISBN: 978-1-4939-7315-6Published: 07 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 234

  • Number of Illustrations: 6 b/w illustrations, 24 illustrations in colour

  • Topics: Plant Sciences

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