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Genomic Mosaicism in Neurons and Other Cell Types

  • Book
  • © 2017

Overview

Editors:
  1. José María Frade

    1. Department of Molecular, Cellular and Developmental Neurobiology, Cajal Institute (IC-CSIC), Madrid, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. Fred H. Gage

    1. Biological Studies and Laboratory of Genetics, The Salk Institute, La Jolla, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 131)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xvi
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Principles and Approaches for Discovery and Validation of Somatic Mosaicism in the Human Brain

      • Alexej Abyzov, Alexander E. Urban, Flora M. Vaccarino
      Pages 3-24

  3. Aneuploidy and Ploidy Variation

    1. Front Matter

      Pages 25-25
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    2. FISH-Based Assays for Detecting Genomic (Chromosomal) Mosaicism in Human Brain Cells

      • Yuri B. Yurov, Svetlana G. Vorsanova, Ilia V. Soloviev, Alexei M. Ratnikov, Ivan Y. Iourov
      Pages 27-41

    3. Flow Cytometric and Sorting Analyses for Nuclear DNA Content, Nucleotide Sequencing, and Interphase FISH

      • Gwendolyn E. Kaeser, Jerold Chun
      Pages 43-55

    4. Flow Cytometric Quantification, Isolation, and Subsequent Epigenetic Analysis of Tetraploid Neurons

      • Noelia López-Sánchez, Iris Patiño-Parrado, José María Frade
      Pages 57-80

    5. A Cytomic Approach Towards Genomic Individuality of Neurons

      • Thomas Arendt, Birgit Belter, Martina K. Brückner, Uwe Ueberham, Markus Morawski, Attila Tarnok
      Pages 81-106

  4. DNA Copy Number Variation

    1. Front Matter

      Pages 107-107
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    2. Single-Cell CNV Detection in Human Neuronal Nuclei

      • Margaret B. Wierman, Ian E. Burbulis, William D. Chronister, Stefan Bekiranov, Michael J. McConnell
      Pages 109-131

    3. Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) for the Analysis of DNA Copy Number Variation

      • Chenghang Zong
      Pages 133-142

    4. Competitive PCR for Copy Number Assessment by Restricting dNTPs

      • Luming Zhou, Robert A. Palais, Yotam Ardon, Carl T. Wittwer
      Pages 143-162

    5. Using Cloning to Amplify Neuronal Genomes for Whole-Genome Sequencing and Comprehensive Mutation Detection and Validation

      • Jennifer L. Hazen, Michael A. Duran, Ryan P. Smith, Alberto R. Rodriguez, Greg S. Martin, Sergey Kupriyanov et al.
      Pages 163-185

  5. LINE-1 Retrotransposition

    1. Front Matter

      Pages 187-187
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    2. Analysis of LINE-1 Retrotransposition in Neural Progenitor Cells and Neurons

      • Angela Macia, Alysson R. Muotri
      Pages 189-208

    3. Estimation of LINE-1 Copy Number in the Brain Tissue and Isolated Neuronal Nuclei

      • Miki Bundo, Tadafumi Kato, Kazuya Iwamoto
      Pages 209-217

    4. Analysis of Somatic LINE-1 Insertions in Neurons

      • Francisco J. Sanchez-Luque, Sandra R. Richardson, Geoffrey J. Faulkner
      Pages 219-251

    5. Single-Cell Whole Genome Amplification and Sequencing to Study Neuronal Mosaicism and Diversity

      • Patrick J. Reed, Meiyan Wang, Jennifer A. Erwin, Apuã C. M. Paquola, Fred H. Gage
      Pages 253-268

  6. Genetic and Genomic Mosaicism in Aging and Disease

    1. Front Matter

      Pages 269-269
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    2. FISH Analysis of Aging-Associated Aneuploidy in Neurons and Nonneuronal Brain Cells

      • Grasiella A. Andriani, Cristina Montagna
      Pages 271-298
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Keywords

About this book

This volume presents methods for the analysis of genomic variability in vertebrate neurons and broadens our knowledge in the ways we understand the brain and its neurons. The chapters in this book are divided into 5 parts, and cover the following topics: principles and approaches for discovery of somatic mosaicism in the brain, aneuploidy and ploidy variation, DNA copy number variation, LINE-1 retrotransposition, and genetic and genomic mosaicism in aging and disease. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Genomic Mosaicism in Neurons and Other Cell Types is a valuable resource for learning about the latest techniques for the analysis of genome and genetic mosaicism in vertebrate neurons.

Editors and Affiliations

  • Department of Molecular, Cellular and Developmental Neurobiology, Cajal Institute (IC-CSIC), Madrid, Spain

    José María Frade

  • Biological Studies and Laboratory of Genetics, The Salk Institute, La Jolla, USA

    Fred H. Gage

Bibliographic Information

  • Book Title: Genomic Mosaicism in Neurons and Other Cell Types

  • Editors: José María Frade, Fred H. Gage

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-7280-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7279-1Published: 09 September 2017

  • Softcover ISBN: 978-1-4939-8440-4Published: 18 August 2018

  • eBook ISBN: 978-1-4939-7280-7Published: 07 September 2017

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XVI, 382

  • Number of Illustrations: 23 b/w illustrations, 67 illustrations in colour

  • Topics: Neurosciences

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