Skip to main content
  • Book
  • © 2018

Brachypodium Genomics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1667)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Brachypodium distachyon Long Noncoding RNAs: Genome-Wide Identification and Expression Analysis

    • Concetta De Quattro, Erica Mica, Mario Enrico Pè, Edoardo Bertolini
    Pages 31-42
  3. A Highly Efficient and Reproducible Fusarium spp. Inoculation Method for Brachypodium distachyon

    • Anuj Rana, Aneesh Karunakaran, Timothy L. Fitzgerald, Rosalie Sabburg, Elizabeth A. B. Aitken, Robert J. Henry et al.
    Pages 43-55
  4. Tissue Culture (Somatic Embryogenesis)-Induced Tnt1 Retrotransposon-Based Mutagenesis in Brachypodium distachyon

    • Upinder S. Gill, Juan C. Serrani-Yarce, Hee-Kyung Lee, Kirankumar S. Mysore
    Pages 57-63
  5. Genomic Approaches to Analyze Alternative Splicing, A Key Regulator of Transcriptome and Proteome Diversity in Brachypodium distachyon

    • Sonia Irigoyen, Renesh H. Bedre, Karen-Beth G. Scholthof, Kranthi K. Mandadi
    Pages 73-85
  6. Molecular Markers in Whole Genome Evolution of Brachypodium

    • Xin-Chun Mo, De-Quan Zhang, Can Kou, Ling-Juan Yin
    Pages 119-137
  7. Estimate Codon Usage Bias Using Codon Usage Analyzer (CUA)

    • Zhenguo Zhang, Gaurav Sablok
    Pages 139-148
  8. Identification of Pseudogenes in Brachypodium distachyon Chromosomes

    • Salvatore Camiolo, Andrea Porceddu
    Pages 149-171
  9. TILLING in Brachypodium distachyon

    • Louise de Bang, Anna Maria Torp, Søren K. Rasmussen
    Pages 173-186
  10. Evaluation of Genome-Wide Markers and Orthologous Markers in Brachypodium distachyon

    • Gaurav Sablok, Suresh B. Mudunuri, Korneliya Gudys, Kranthi Chennamsetti, G. P. Saradhi Varma, Miroslaw Kwasniewski
    Pages 195-201

About this book

This volume presents protocols for Brachypodium genomics in numerous areas ranging from marker development, trait evolution, functional genomics, metabolomics, transcriptomics, genomics, and tilling. This book also explores techniques to study the widening genetic base of Brachypodium that will help researchers better understand the model plant using NGS technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Brachypodium Genomics: Methods and Protocols is a valuable resource for bench-oriented molecular biologists and computational biologists working towards further evolving this field.

Editors and Affiliations

  • Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach IASMA, Trentino, Italy

    Gaurav Sablok

  • Sabanci University, Istanbul, Turkey

    Hikmet Budak

  • Climate Change Cluster (C3), University of Technology Sydney, Sydney, Australia

    Peter J. Ralph

Bibliographic Information

  • Book Title: Brachypodium Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Gaurav Sablok, Hikmet Budak, Peter J. Ralph

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7278-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7276-0Published: 20 October 2017

  • Softcover ISBN: 978-1-4939-8439-8Published: 18 May 2018

  • eBook ISBN: 978-1-4939-7278-4Published: 16 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 312

  • Number of Illustrations: 17 b/w illustrations, 52 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access