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  • © 2017

Plant Protein Secretion

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1662)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
  2. An Overview of Protein Secretion in Yeast and Animal Cells

    • Yusong Guo, Feng Yang, Xiao Tang
    Pages 1-17
  3. An Overview of Protein Secretion in Plant Cells

    • Kin Pan Chung, Yonglun Zeng
    Pages 19-32
  4. Proteomic Analysis of Secreted Proteins from Cell Microenvironment

    • Subash Adhikari, Lan Chen, Peiwu Huang, Ruijun Tian
    Pages 45-58
  5. Analysis of Golgi-Mediated Protein Traffic in Plant Cells

    • Wenjin Shen, Zhidan Xiao, Jinbo Shen, Caiji Gao
    Pages 75-86
  6. Analysis of Membrane Protein Topology in the Plant Secretory Pathway

    • Jinya Guo, Yansong Miao, Yi Cai
    Pages 87-95
  7. 3D Printing of Plant Golgi Stacks from Their Electron Tomographic Models

    • Keith Ka Ki Mai, Madison J. Kang, Byung-Ho Kang
    Pages 105-113
  8. Analysis of Actin-Based Intracellular Trafficking in Pollen Tubes

    • Yuxiang Jiang, Meng Zhang, Shanjin Huang
    Pages 125-136
  9. Analysis of Phragmoplast Kinetics During Plant Cytokinesis

    • Pantelis Livanos, Mayank Chugh, Sabine Müller
    Pages 137-150
  10. Measuring Plant Protein Secretion

    • Emily R. Larson
    Pages 199-207

About this book

This volume aims to provide an update on recent developments in protein secretion studies in plants versus yeast and mammalian systems. This book also discusses case studies that analyze the use of plant protein secretion using various tools and systems. The chapters in this book explore topics such as the study of Golgi-mediated protein traffic in plant cells; actin-based intracellular trafficking in pollen tubes; secretion system for identification of cargo proteins of vacuolar sorting receptors; isolation of the plant exocyst complex; and plant autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Plant Protein Secretion: Methods and Protocols is a valuable resource for researchers interested in furthering their studies in plant protein secretion."

Editors and Affiliations

  • School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China

    Liwen Jiang

Bibliographic Information

  • Book Title: Plant Protein Secretion

  • Book Subtitle: Methods and Protocols

  • Editors: Liwen Jiang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7262-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7261-6Published: 02 September 2017

  • Softcover ISBN: 978-1-4939-8434-3Published: 03 August 2018

  • eBook ISBN: 978-1-4939-7262-3Published: 31 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 285

  • Number of Illustrations: 8 b/w illustrations, 60 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access