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Extracellular Vesicles

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Winston Patrick Kuo

    1. CloudHealth Genomics, Ltd., Shanghai, China

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Shidong Jia

    1. Predicine, Inc., Hayward, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of extracellular vesicles
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1660)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine

    • Mingyi Shang, John S. Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo et al.
    Pages 1-14

  3. Red Blood Cells: A Source of Extracellular Vesicles

    • Winston Patrick Kuo, John C. Tigges, Vasilis Toxavidis, Ionita Ghiran
    Pages 15-22

  4. Isolation of Extracellular Vesicles

    1. Front Matter

      Pages 23-23
      Download chapter PDF

    2. Isolation of Extracellular Vesicles by Ultracentrifugation

      • Fatemeh Momen-Heravi
      Pages 25-32

    3. Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles

      • Mitja L. Heinemann, Jody Vykoukal
      Pages 33-41

    4. Paper-Based for Isolation of Extracellular Vesicles

      • Yi-Hsing Hsiao, Chihchen Chen
      Pages 43-54

    5. Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis

      • Cindy M. Yamamoto, Taku Murakami, Shu-Wing Ng
      Pages 55-63

    6. Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads

      • Ketil W. Pedersen, Bente Kierulf, Axl Neurauter
      Pages 65-87

  5. Purification of Extracellular Vesicles

    1. Front Matter

      Pages 89-89
      Download chapter PDF

    2. Polymer-Based Purification of Extracellular Vesicles

      • Peter N. Brown, Hang Yin
      Pages 91-103

    3. Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification

      • Richard Lobb, Andreas Möller
      Pages 105-110

    4. Purification Protocols for Extracellular Vesicles

      • Rebecca E. Lane, Darren Korbie, Matt Trau, Michelle M. Hill
      Pages 111-130

  6. Characterization of Extracellular Vesicles

    1. Front Matter

      Pages 131-131
      Download chapter PDF

    2. Characterization of Extracellular Vesicles by Surface Plasmon Resonance

      • Hyungsoon Im, Katherine Yang, Hakho Lee, Cesar M. Castro
      Pages 133-141

    3. Extracellular Vesicle Isolation and Analysis by Western Blotting

      • Emma J. K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, George M. Church
      Pages 143-152

    4. Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis

      • Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, Rebecca Dragovic
      Pages 153-173

    5. Characterization of Extracellular Vesicles by Flow Cytometry

      • Virginia Camacho, Vasilis Toxavidis, John C. Tigges
      Pages 175-190

    6. Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC)

      • Tao Huang, Jiang He
      Pages 191-199

    7. Multi-Surface Antigen Staining of Larger Extracellular Vesicles

      • Veronika Lukacs-Kornek, Henrike Julich-Haertel, Sabine Katharina Urban, Miroslaw Kornek
      Pages 201-208
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Keywords

About this book

This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who have contributed to this collection provide a variety of different techniques related to the growing assortment of EV applications, given that at times using only one technique or two is insufficient to address the question at hand. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Extracellular Vesicles: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of EV functions and applications.

Editors and Affiliations

  • CloudHealth Genomics, Ltd., Shanghai, China

    Winston Patrick Kuo

  • Predicine, Inc., Hayward, USA

    Shidong Jia

Bibliographic Information

  • Book Title: Extracellular Vesicles

  • Book Subtitle: Methods and Protocols

  • Editors: Winston Patrick Kuo, Shidong Jia

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7253-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-7251-7Published: 21 August 2017

  • Softcover ISBN: 978-1-4939-8431-2Published: 03 August 2018

  • eBook ISBN: 978-1-4939-7253-1Published: 21 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 446

  • Number of Illustrations: 40 b/w illustrations, 72 illustrations in colour

  • Topics: Cell Biology

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