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  • © 2018

Biochemical Approaches for Glutamatergic Neurotransmission

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 130)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xxii
  2. Identification of Glutamatergic Neurons

    • Shiliang Zhang, Marisela Morales
    Pages 1-28
  3. Imaging Glutamate with Genetically Encoded Fluorescent Sensors

    • Gerard J. Broussard, Elizabeth K. Unger, Ruqiang Liang, Brian P. McGrew, Lin Tian
    Pages 117-153
  4. Measurement of Astrocytic Glutamate Release Using Genetically Encoded Probe Combined with TIRF Microscopy

    • Fuyuko Takata-Tsuji, Naura Choulnamountri, Christophe Place, Olivier Pascual
    Pages 155-168
  5. In Vivo Electrochemical Studies of Optogenetic Control of Glutamate Signaling Measured Using Enzyme-Based Ceramic Microelectrode Arrays

    • Jason J. Burmeister, Francois Pomerleau, Jorge E. Quintero, Peter Huettl, Yi Ai, Johan Jakobsson et al.
    Pages 327-351
  6. In Vivo Determination of Glutamate Uptake by Brain Microdialysis

    • Sandrine Parrot, Monique Touret, Luc Denoroy
    Pages 431-467
  7. Enzymes of Glutamate System

    • Irina S. Boksha, Olga K. Savushkina, Elena B. Tereshkina, Tatyana A. Prokhorova, Elizabeta B. Mukaetova-Ladinska
    Pages 469-506
  8. Clinical CNS Microdialysis of Glutamate with a Special Methodological Focus on Human Spinal Cord

    • Bernard Renaud, Luc Denoroy, Delphine Collin-Chavagnac, Patrick Mertens, Sandrine Parrot
    Pages 523-558
  9. Back Matter

    Pages 559-565

About this book

This volume presents techniques and recent developments in biochemical approaches to study glutamatergic neurotransmission. This book contains detailed discussions on tracing neuronal pathways, functional or spectroscopic imaging, optogenetic or pharmacological tools, and extracellular neurochemistry in experimental clinical models. The chapters cover topics such as: identification of glutamatergic neurons in a discrete brain area, tools to study dynamics of single ionotropic receptors, in vivo extracellular glutamate monitoring in preclinical research using biosensors, capillary electrophoresis and liquid chromatography, and potential brain glutamatergic clinical biomarkers in biofluids and tissues. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.


Cutting-edge and authoritative, Biochemical Approaches for Glutamatergic Neurotransmission is a valuable resource for researchers studying glutamate neurochemistry.

Editors and Affiliations

  • Lyon Neuroscience Research Center, University Lyon 1, Lyon, France

    Sandrine Parrot, Luc Denoroy

Bibliographic Information

  • Book Title: Biochemical Approaches for Glutamatergic Neurotransmission

  • Editors: Sandrine Parrot, Luc Denoroy

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-7228-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7227-2Published: 22 September 2017

  • Softcover ISBN: 978-1-4939-8423-7Published: 18 August 2018

  • eBook ISBN: 978-1-4939-7228-9Published: 21 September 2017

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XXII, 565

  • Number of Illustrations: 35 b/w illustrations, 101 illustrations in colour

  • Topics: Neurosciences

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access