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Avian and Reptilian Developmental Biology

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Guojun Sheng

    1. IRCMS, Kumamoto University, Kumamoto, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1650)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Some Thoughts on Experimental Design

    • Claudio D. Stern
    Pages 1-8

  3. Genomics and Transcriptomics

    1. Front Matter

      Pages 9-9
      Download chapter PDF

    2. Comparative Genomics as a Foundation for Evo-Devo Studies in Birds

      • Phil Grayson, Simon Y. W. Sin, Timothy B. Sackton, Scott V. Edwards
      Pages 11-46

    3. A Step-by-Step Guide to Assemble a Reptilian Genome

      • Asier Ullate-Agote, Yingguang Frank Chan, Athanasia C. Tzika
      Pages 47-67

    4. Genomic and Transcriptomic Analyses of Avian Sex Chromosomes and Sex-Linked Genes

      • Jilin Zhang, Jing Li, Qi Zhou
      Pages 69-85

    5. Systems Biology Analyses in Chicken: Workflow for Transcriptome and ChIP-Seq Analyses Using the Chicken Skin Paradigm

      • Yung-Chih Lai, Randall B. Widelitz, Cheng-Ming Chuong
      Pages 87-100

    6. Application of a CAGE Method to an Avian Development Study

      • Ruslan Deviatiiarov, Marina Lizio, Oleg Gusev
      Pages 101-109

  4. Genetic Manipulation

    1. Front Matter

      Pages 111-111
      Download chapter PDF

    2. CRISPR/Cas9 in the Chicken Embryo

      • Valérie Morin, Nadège Véron, Christophe Marcelle
      Pages 113-123

    3. Fluorescent Quail: A Transgenic Model System for the Dynamic Study of Avian Development

      • David Huss, Rusty Lansford
      Pages 125-147

    4. Lentiviral-Mediated Transgenesis in Songbirds

      • Wan-chun Liu, Marian Hruska-Plochan, Atsushi Miyanohara
      Pages 149-165

    5. In Ovo Electroporation Methods in Chick Embryos

      • Hidekiyo Harada, Minoru Omi, Harukazu Nakamura
      Pages 167-176

    6. Genetic Manipulation of the Avian Urogenital System Using In Ovo Electroporation

      • Claire E. Hirst, Olivier Serralbo, Katie L. Ayers, Kelly N. Roeszler, Craig A. Smith
      Pages 177-190

    7. Enhancer Analyses Using Chicken Embryo Electroporation

      • Masanori Uchikawa, Naoko Nishimura, Makiko Iwafuchi-Doi, Hisato Kondoh
      Pages 191-202

    8. Transgene Introduction into the Chick Limb Bud by Electroporation

      • Shogo Ueda, Takayuki Suzuki, Mikiko Tanaka
      Pages 203-208

  5. Stem Cells

    1. Front Matter

      Pages 209-209
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    2. Chicken Induced Pluripotent Stem Cells: Establishment and Characterization

      • Aurelie Fuet, Bertrand Pain
      Pages 211-228

    3. Isolation and Characterization of Chicken Primordial Germ Cells and Their Application in Transgenesis

      • Jae Yong Han, Bo Ram Lee
      Pages 229-242

    4. Handling of Gametes for In Vitro Insemination in Birds

      • Shusei Mizushima, Mei Matsuzaki, Tomohiro Sasanami
      Pages 243-257
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Keywords

About this book

This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic manipulation; Part III focuses on methods to handle pluripotent cells; and part IV looks at the emerging models in avian and reptilian developmental biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Avian and Reptilian Developmental Biology: Methods and Protocols explores a var

iety of approaches and different sauropsid models that will help facilitate communication and collaboration among researchers, which in turn will progress this field forward.

Editors and Affiliations

  • IRCMS, Kumamoto University, Kumamoto, Japan

    Guojun Sheng

Bibliographic Information

  • Book Title: Avian and Reptilian Developmental Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Guojun Sheng

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7216-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7215-9Published: 15 August 2017

  • Softcover ISBN: 978-1-4939-8419-0Published: 03 August 2018

  • eBook ISBN: 978-1-4939-7216-6Published: 14 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 365

  • Number of Illustrations: 16 b/w illustrations, 75 illustrations in colour

  • Topics: Cell Biology

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