Skip to main content
  • Book
  • © 2017

Avian and Reptilian Developmental Biology

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1650)

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
  2. Some Thoughts on Experimental Design

    • Claudio D. Stern
    Pages 1-8
  3. Genomics and Transcriptomics

    1. Front Matter

      Pages 9-9
    2. Comparative Genomics as a Foundation for Evo-Devo Studies in Birds

      • Phil Grayson, Simon Y. W. Sin, Timothy B. Sackton, Scott V. Edwards
      Pages 11-46
    3. A Step-by-Step Guide to Assemble a Reptilian Genome

      • Asier Ullate-Agote, Yingguang Frank Chan, Athanasia C. Tzika
      Pages 47-67
    4. Application of a CAGE Method to an Avian Development Study

      • Ruslan Deviatiiarov, Marina Lizio, Oleg Gusev
      Pages 101-109
  4. Genetic Manipulation

    1. Front Matter

      Pages 111-111
    2. CRISPR/Cas9 in the Chicken Embryo

      • Valérie Morin, Nadège Véron, Christophe Marcelle
      Pages 113-123
    3. Lentiviral-Mediated Transgenesis in Songbirds

      • Wan-chun Liu, Marian Hruska-Plochan, Atsushi Miyanohara
      Pages 149-165
    4. In Ovo Electroporation Methods in Chick Embryos

      • Hidekiyo Harada, Minoru Omi, Harukazu Nakamura
      Pages 167-176
    5. Genetic Manipulation of the Avian Urogenital System Using In Ovo Electroporation

      • Claire E. Hirst, Olivier Serralbo, Katie L. Ayers, Kelly N. Roeszler, Craig A. Smith
      Pages 177-190
    6. Enhancer Analyses Using Chicken Embryo Electroporation

      • Masanori Uchikawa, Naoko Nishimura, Makiko Iwafuchi-Doi, Hisato Kondoh
      Pages 191-202
    7. Transgene Introduction into the Chick Limb Bud by Electroporation

      • Shogo Ueda, Takayuki Suzuki, Mikiko Tanaka
      Pages 203-208
  5. Stem Cells

    1. Front Matter

      Pages 209-209
    2. Handling of Gametes for In Vitro Insemination in Birds

      • Shusei Mizushima, Mei Matsuzaki, Tomohiro Sasanami
      Pages 243-257

About this book

This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic manipulation; Part III focuses on methods to handle pluripotent cells; and part IV looks at the emerging models in avian and reptilian developmental biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Avian and Reptilian Developmental Biology: Methods and Protocols explores a var

iety of approaches and different sauropsid models that will help facilitate communication and collaboration among researchers, which in turn will progress this field forward.

Editors and Affiliations

  • IRCMS, Kumamoto University, Kumamoto, Japan

    Guojun Sheng

Bibliographic Information

  • Book Title: Avian and Reptilian Developmental Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Guojun Sheng

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7216-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7215-9Published: 15 August 2017

  • Softcover ISBN: 978-1-4939-8419-0Published: 03 August 2018

  • eBook ISBN: 978-1-4939-7216-6Published: 14 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 365

  • Number of Illustrations: 16 b/w illustrations, 75 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access