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Natural Antibodies

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Srinivas V. Kaveri

    1. Centre de Recherche des Cordeliers, INSERM, PARIS, Paris, France

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    You can also search for this editor in PubMed   Google Scholar

  2. Jagadeesh Bayry

    1. Centre de Recherche des Cordeliers, INSERM, PARIS, Paris, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1643)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
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  2. Natural Antibodies: Next Steps Toward Translational Investigation

    • Heinz Kohler
    Pages 1-3

  3. Isolation of Natural Anti-FcεRIα Autoantibodies from Healthy Donors

    • Monique Vogel, Michael P. Horn
    Pages 5-22

  4. Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid

    • Christoph Schneider, Marlies Illi, Marius Lötscher, Marc Wehrli, Stephan von Gunten
    Pages 23-31

  5. Purification of Natural Antibodies Against Tau Protein by Affinity Chromatography

    • Michala Krestova, Lenka Hromadkova, Jan Ricny
    Pages 33-44

  6. Unbiased RACE-Based Massive Parallel Surveys of Human IgA Antibody Repertoires

    • Hanane El Bannoudi, Céline Anquetil, Marc J. Braunstein, Sergei L. Kosakovsky Pond, Gregg J. Silverman
    Pages 45-73

  7. Analysis of Signaling Events in B-1a Cells

    • Jianxin Huo, Yuhan Huang, Shengli Xu, Kong-Peng Lam
    Pages 75-83

  8. Exploring the Role of Microbiota in the Limiting of B1 and MZ B-Cell Numbers by Naturally Secreted Immunoglobulins

    • Elodie Mohr, Andreia C. Lino
    Pages 85-103

  9. Assessment of Anti-Tumor Cytotoxic Activity of Naturally Occurring Antibodies in Human Serum or Plasma

    • Reinhard Schwartz-Albiez, Othmar Dill
    Pages 105-110

  10. Hydrolysis and Dissolution of Amyloids by Catabodies

    • Ville V. Meretoja, Sudhir Paul, Stephanie A. Planque
    Pages 111-134

  11. Methods for Posttranslational Induction of Polyreactivity of Antibodies

    • Maxime Lecerf, Annaelle Jarossay, Srinivas V. Kaveri, Sébastien Lacroix-Desmazes, Jordan D. Dimitrov
    Pages 135-145

  12. Characterization of Natural IgM Antibodies Recognizing Oxidation-Specific Epitopes on Circulating Microvesicles

    • Florian Puhm, Christoph J. Binder
    Pages 147-154

  13. Natural Monoclonal Antibody to Oxidized Low-Density Lipoprotein and Aggregatibacter actinomycetemcomitans

    • Chunguang Wang, Sohvi Hörkkö
    Pages 155-167

  14. Detection of Natural Antibodies and Serological Diagnosis of Pneumococcal Pneumonia Using a Bead-Based High-Throughput Assay

    • Irene Jiménez-Munguía, Willem J. B. van Wamel, Manuel J. Rodríguez-Ortega, Ignacio Obando
    Pages 169-177

  15. Detection of Naturally Occurring Human Antibodies Against Gangliosides by ELISA

    • Ana María Hernández, Nely Rodríguez-Zhurbenko
    Pages 179-186

  16. Evaluating the Impact of Natural IgM on Adenovirus Type 5 Gene Therapy Vectors

    • Zhili Xu, Jie Tian, Andrew W. Harmon, Andrew P. Byrnes
    Pages 187-196

  17. Erratum to: Hydrolysis and Dissolution of Amyloids by Catabodies

    • Ville V. Meretoja, Sudhir Paul, Stephanie A. Planque
    Pages E1-E1

  18. Back Matter

    Pages 197-199
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Keywords

About this book

This volume looks at the role of natural antibodies in pathogen elimination, cell survival, inflammation, cancer, and autoimmunity. The chapters in this book cover numerous topics, such as isolation of natural antibodies; methods for separating natural antibodies from human plasma, saliva, breast milk, and gastrointestinal fluids; functional properties of natural antibodies such as  anti-tumor cytotoxic activity, and hydrolysis and dissolution of their target antigens; their utility in serological diagnosis of microbial antigens; and the role of natural antibodies in inhibiting viral vectors in the absence of prior exposure to the virus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Natural Antibodies: Methods and Protocols is a valuable resource for researchers and experts who are interested in further studies of natural antibodies.

Editors and Affiliations

  • Centre de Recherche des Cordeliers, INSERM, PARIS, Paris, France

    Srinivas V. Kaveri, Jagadeesh Bayry

Bibliographic Information

  • Book Title: Natural Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Srinivas V. Kaveri, Jagadeesh Bayry

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7180-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7179-4Published: 01 July 2017

  • Softcover ISBN: 978-1-4939-8409-1Published: 12 May 2018

  • eBook ISBN: 978-1-4939-7180-0Published: 30 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 199

  • Number of Illustrations: 11 b/w illustrations, 11 illustrations in colour

  • Topics: Immunology

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