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Kinase Signaling Networks

  • Book
  • © 2017

Overview

Editors:
  1. Aik-Choon Tan

    1. Division of Medical Oncology, University of Colorado, Aurora, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Paul H. Huang

    1. Protein Networks Laboratory, Institute of Cancer Research, London, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of kinase signaling networks
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
  • Includes supplementary material: sn.pub/extras
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1636)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Synthetic Biology, Chemical Biology, and Screening Approaches

    1. Front Matter

      Pages 1-1
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    2. Optogenetic Control of Ras/Erk Signaling Using the Phy–PIF System

      • Alexander G. Goglia, Maxwell Z. Wilson, Daniel B. DiGiorno, Jared E. Toettcher
      Pages 3-20

    3. Dissecting Kinase Effector Signaling Using the RapRTAP Methodology

      • Anne-Marie Ray, Jennifer E. Klomp, Kerrie B. Collins, Andrei V. Karginov
      Pages 21-33

    4. Single-Cell Imaging of ERK Signaling Using Fluorescent Biosensors

      • Michael Pargett, Taryn E. Gillies, Carolyn K. Teragawa, Breanne Sparta, John G. Albeck
      Pages 35-59

    5. Quantification of Cell Signaling Networks Using Kinase Activity Chemosensors

      • Jon R. Beck, Edward N. Harris, Cliff I. Stains
      Pages 61-70

    6. Expression of Recombinant Phosphoproteins for Signal Transduction Studies

      • Karl W. Barber, Jesse Rinehart
      Pages 71-78

    7. Allosteric Modulation of Src Family Kinases with ATP-Competitive Inhibitors

      • Ames C. Register, Sujata Chakraborty, Dustin J. Maly
      Pages 79-89

    8. Characterization of Ligand Binding to Pseudokinases Using a Thermal Shift Assay

      • Isabelle S. Lucet, James M. Murphy
      Pages 91-104

    9. Proteomic Profiling of Protein Kinase Inhibitor Targets by Mass Spectrometry

      • Martin Golkowski, Dustin J. Maly, Shao-En Ong
      Pages 105-117

    10. Utilizing the Luminex Magnetic Bead-Based Suspension Array for Rapid Multiplexed Phosphoprotein Quantification

      • Adam Stewart, Udai Banerji
      Pages 119-131

    11. High-Content Imaging and RNAi Screens for Investigating Kinase Network Plasticity

      • Simon R. Stockwell, Sibylle Mittnacht
      Pages 133-161

    12. Analysis of Drug Resistance Using Kinome-Wide Functional Screens

      • Katherine R. Singleton, Keith T. Earley, Lynn E. Heasley
      Pages 163-177

    13. Identification and Validation of Driver Kinases from Next-Generation Sequencing Data

      • Andri Leonidou, Barrie Peck, Rachael Natrajan
      Pages 179-195

  3. Mass Spectrometry and Metabolic Analysis of Kinase Signaling

    1. Front Matter

      Pages 197-197
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    2. Label-Free Phosphoproteomic Approach for Kinase Signaling Analysis

      • Edmund Wilkes, Pedro R. Cutillas
      Pages 199-217

    3. Cell-Specific Labeling for Analyzing Bidirectional Signaling by Mass Spectrometry

      • Christopher J. Tape, Claus Jørgensen
      Pages 219-234

    4. Characterization of the Phospho-Adhesome by Mass Spectrometry-Based Proteomics

      • Joseph Robertson, Jonathan D. Humphries, Nikki R. Paul, Stacey Warwood, David Knight, Adam Byron et al.
      Pages 235-251

    5. Analysis of Phosphotyrosine Signaling Networks in Lung Cancer Cell Lines

      • Malgorzata Broncel, Paul H. Huang
      Pages 253-262

    6. Targeted Analysis of Phosphotyrosine Signaling by Multiple Reaction Monitoring Mass Spectrometry

      • Leo S. Payne, Paul H. Huang
      Pages 263-281
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Keywords

About this book

This detailed volume describes cutting-edge techniques in three distinct and complementary areas of contemporary kinase biology research. Beginning with a section on synthetic biology, chemical biology, and screening approaches to kinase signaling networks, the book continues with sections on mass spectrometry and metabolic analysis of kinase signaling as well as computational analysis of kinase signaling networks. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Kinase Signaling Networks aims to provide scientists with the tools necessary to overcome the technological bottlenecks that limit our ability to gain a deeper mechanistic understanding of kinase biology.

Editors and Affiliations

  • Division of Medical Oncology, University of Colorado, Aurora, USA

    Aik-Choon Tan

  • Protein Networks Laboratory, Institute of Cancer Research, London, United Kingdom

    Paul H. Huang

Bibliographic Information

  • Book Title: Kinase Signaling Networks

  • Editors: Aik-Choon Tan, Paul H. Huang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7154-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-7152-7Published: 21 July 2017

  • Softcover ISBN: 978-1-4939-8400-8Published: 28 July 2018

  • eBook ISBN: 978-1-4939-7154-1Published: 19 July 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 537

  • Number of Illustrations: 38 b/w illustrations, 74 illustrations in colour

  • Topics: Biochemistry, general

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