Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1634)
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Table of contents (24 protocols)
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Front Matter
About this book
This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Circula
Editors and Affiliations
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Division of Hematology Oncology, Helen Diller Family Comprehensive Cancer Center, University of California-San Francisco, San Francisco, USA
Mark Jesus M. Magbanua, John W. Park
Bibliographic Information
Book Title: Circulating Tumor Cells
Book Subtitle: Methods and Protocols
Editors: Mark Jesus M. Magbanua, John W. Park
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7144-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-7143-5Published: 18 August 2017
Softcover ISBN: 978-1-4939-8397-1Published: 03 August 2018
eBook ISBN: 978-1-4939-7144-2Published: 17 August 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XXV, 306
Number of Illustrations: 21 b/w illustrations, 75 illustrations in colour
Topics: Cancer Research