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  • © 2017

Plant Receptor Kinases

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential fro reproducible methods
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1621)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
  2. Expression and Purification of PRKs

    1. Front Matter

      Pages 1-1
    2. Expression of Plant Receptor Kinases in E. coli

      • Moneeza Akbar Agha, David Lightfoot, Ahmed Jawaad Afzal
      Pages 3-20
    3. Expression of Plant Receptor Kinases in Yeast

      • María Laura Barberini, Jorge P. Muschietti
      Pages 21-27
    4. Expression of Plant Receptor Kinases in Tobacco BY-2 Cells

      • Hidefumi Shinohara, Yoshikatsu Matsubayashi
      Pages 29-35
    5. Cell-Free Synthesis of Plant Receptor Kinases

      • Akira Nozawa, Keiichirou Nemoto, Shunsuke Nomura, Satoshi Yamanaka, Kohki Kido, Tatsuya Sawasaki
      Pages 37-46
  3. Ligand Binding and Activation of PRKs

    1. Front Matter

      Pages 57-57
    2. Photoaffinity Labeling of Plant Receptor Kinases

      • Hidefumi Shinohara, Yoshikatsu Matsubayashi
      Pages 59-68
  4. In silico Methods for Identification and Analyses of PRKs

    1. Front Matter

      Pages 77-77
    2. Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis

      • Aleksia Vaattovaara, Jarkko Salojärvi, Michael Wrzaczek
      Pages 79-91
  5. Identification of Enzymatic Functions and Interacting Partners of PRKs

    1. Front Matter

      Pages 107-107
    2. Co-immunoprecipitation of Plant Receptor Kinases

      • María Laura Barberini, Jorge P. Muschietti
      Pages 109-112
    3. Autophosphorylation Assays Using Plant Receptor Kinases Synthesized in Cell-Free Systems

      • Keiichirou Nemoto, Akira Nozawa, Satoshi Yamanaka, Shunsuke Nomura, Kohki Kido, Tatsuya Sawasaki
      Pages 113-120
    4. BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases

      • Cornelia Fischer, Margret Sauter, Petra Dietrich
      Pages 141-149

About this book

This is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways. Written in the highly successful Methods

in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Receptor Kinases: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Biosciences, University of Oslo, Oslo, Norway

    Reidunn Birgitta Aalen

Bibliographic Information

  • Book Title: Plant Receptor Kinases

  • Book Subtitle: Methods and Protocols

  • Editors: Reidunn Birgitta Aalen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7063-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7062-9Published: 01 June 2017

  • Softcover ISBN: 978-1-4939-8382-7Published: 08 August 2018

  • eBook ISBN: 978-1-4939-7063-6Published: 31 May 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 196

  • Number of Illustrations: 23 b/w illustrations, 18 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access