This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protocols that can be applied to diverse or even unknown pathogens, digital PCR, next generation sequencing, and bioinformatic analyses. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Diagnostic Bacteriology: Methods and Protocols delivers a widerange of assay types all on the cutting edge of diagnostic bacteriology.

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Editors and Affiliations

Germantown, USA

Kimberly A. Bishop-Lilly

Bibliographic Information

Book Title: Diagnostic Bacteriology
Book Subtitle: Methods and Protocols
Editors: Kimberly A. Bishop-Lilly
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7037-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-7035-3Published: 10 June 2017
Softcover ISBN: 978-1-4939-8375-9Published: 12 August 2018
eBook ISBN: 978-1-4939-7037-7Published: 09 June 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 264
Number of Illustrations: 12 b/w illustrations, 22 illustrations in colour
Topics: Microbiology

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Table of contents (17 protocols)

Front Matter

Whole-Genome Enrichment Using RNA Probes and Sequencing of Chlamydia trachomatis Directly from Clinical Samples

Characterization of Sinus Microbiota by 16S Sequencing from Swabs

Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR)

Detection of Helicobacter pylori DNA in Formalin-Fixed Paraffin-Embedded Gastric Biopsies Using Laser Microdissection and qPCR

Mycobacterial Load Assay

Defining Diagnostic Biomarkers Using Shotgun Proteomics and MALDI-TOF Mass Spectrometry

Detection and Typing of “Candidatus Phytoplasma ” spp. in Host DNA Extracts Using Oligonucleotide-Coupled Fluorescent Microspheres

Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization

Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections

Detection and Differentiation of Lyme Spirochetes and Other Tick-Borne Pathogens from Blood Using Real-Time PCR with Molecular Beacons

Methods for Real-Time PCR-Based Diagnosis of Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia abortus Infections in an Opened Molecular Diagnostic Platform

Real-Time PCR to Identify Staphylococci and Assay for Virulence from Blood

Multiplex Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis

A Closed-tube Loop-Mediated Isothermal Amplification Assay for the Visual Endpoint Detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis

Highly Specific Ligation-dependent Microarray Detection of Single Nucleotide Polymorphisms

Multilocus Sequence Typing (MLST) for Cronobacter spp.