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Diagnostic Bacteriology

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Kimberly A. Bishop-Lilly

    1. Germantown, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1616)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Whole-Genome Enrichment Using RNA Probes and Sequencing of Chlamydia trachomatis Directly from Clinical Samples

    • Amanda Claire Brown, Mette T. Christiansen
    Pages 1-22

  3. Characterization of Sinus Microbiota by 16S Sequencing from Swabs

    • Thad W. Vickery, Jennifer M. Kofonow, Vijay R. Ramakrishnan
    Pages 23-38

  4. Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR)

    • Véronique Wuyts, Wesley Mattheus, Nancy H. C. Roosens, Kathleen Marchal, Sophie Bertrand, Sigrid C. J. De Keersmaecker
    Pages 39-69

  5. Detection of Helicobacter pylori DNA in Formalin-Fixed Paraffin-Embedded Gastric Biopsies Using Laser Microdissection and qPCR

    • María Fernanda Loayza Villa, Valeria Liliana Herrera Sevilla, Nicolás Vivar-Diaz
    Pages 71-88

  6. Mycobacterial Load Assay

    • Stephen H. Gillespie, Wilber Sabiiti, Katarina Oravcova
    Pages 89-105

  7. Defining Diagnostic Biomarkers Using Shotgun Proteomics and MALDI-TOF Mass Spectrometry

    • Jean Armengaud
    Pages 107-120

  8. Detection and Typing of “Candidatus Phytoplasma ” spp. in Host DNA Extracts Using Oligonucleotide-Coupled Fluorescent Microspheres

    • Edel Pérez-López, Christine Hammond, Chrystel Olivier, Tim J. Dumonceaux
    Pages 121-136

  9. Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization

    • Silvia Fontenete, Marina Leite, Ceu Figueiredo, Paul Cos, Nuno F. Azevedo
    Pages 137-146

  10. Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections

    • Anja Mezger, Mats Nilsson, Dan I. Andersson
    Pages 147-153

  11. Detection and Differentiation of Lyme Spirochetes and Other Tick-Borne Pathogens from Blood Using Real-Time PCR with Molecular Beacons

    • Samantha Schlachter, Kamfai Chan, Salvatore A. E. Marras, Nikhat Parveen
    Pages 155-170

  12. Methods for Real-Time PCR-Based Diagnosis of Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia abortus Infections in an Opened Molecular Diagnostic Platform

    • Onya Opota, René Brouillet, Gilbert Greub, Katia Jaton
    Pages 171-181

  13. Real-Time PCR to Identify Staphylococci and Assay for Virulence from Blood

    • Charles E. Okolie
    Pages 183-207

  14. Multiplex Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis

    • Antonio Machado, Nuno Cerca
    Pages 209-219

  15. A Closed-tube Loop-Mediated Isothermal Amplification Assay for the Visual Endpoint Detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis

    • Marcos D. Trangoni, Andrea K. Gioffré, Silvio L. Cravero
    Pages 221-229

  16. Highly Specific Ligation-dependent Microarray Detection of Single Nucleotide Polymorphisms

    • Noa Wolff, Ivan Barišicʹ
    Pages 231-240

  17. Multilocus Sequence Typing (MLST) for Cronobacter spp.

    • Susan Joseph, Stephen Forsythe
    Pages 241-248

  18. Diagnostic Bacteriology: Raman Spectroscopy

    • Rebecca L. Pavlicek, Nicole J. Crane, Meron Ghebremedhin, Katherine E. Cilwa, Eric A. Elster
    Pages 249-261

  19. Back Matter

    Pages 263-263
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Keywords

About this book

This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protocols that can be applied to diverse or even unknown pathogens, digital PCR, next generation sequencing, and bioinformatic analyses. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Diagnostic Bacteriology: Methods and Protocols delivers a widerange of assay types all on the cutting edge of diagnostic bacteriology.

Editors and Affiliations

  • Germantown, USA

    Kimberly A. Bishop-Lilly

Bibliographic Information

  • Book Title: Diagnostic Bacteriology

  • Book Subtitle: Methods and Protocols

  • Editors: Kimberly A. Bishop-Lilly

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7037-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7035-3Published: 10 June 2017

  • Softcover ISBN: 978-1-4939-8375-9Published: 12 August 2018

  • eBook ISBN: 978-1-4939-7037-7Published: 09 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 264

  • Number of Illustrations: 12 b/w illustrations, 22 illustrations in colour

  • Topics: Microbiology

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