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3D Cell Culture

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Zuzana Koledova

    1. Department of Histology and Embryology, Masaryk University, Brno, Czech Republic

    View editor publications

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  • Includes cutting-edge methods and protocols for the study of 3D cell cultures
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1612)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. 3D Cell Culture: An Introduction

    • Zuzana Koledova
    Pages 1-11

  3. Hydrogels and Scaffolds for 3D Cell Culture

    1. Front Matter

      Pages 13-13
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    2. Preparation of Decellularized Biological Scaffolds for 3D Cell Culture

      • Bryan N. Brown, Michael J. Buckenmeyer, Travis A. Prest
      Pages 15-27

    3. 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix

      • Katrina Wisdom, Ovijit Chaudhuri
      Pages 29-37

    4. Hydrogel-Based In Vitro Models of Tumor Angiogenesis

      • Laura J. Bray, Marcus Binner, Uwe Freudenberg, Carsten Werner
      Pages 39-63

    5. Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional Hydrogels

      • Massimiliano Caiazzo, Yoji Tabata, Matthias P. Lutolf
      Pages 65-78

    6. Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modeling in Three Dimensions

      • Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, Maria-Pau Ginebra
      Pages 79-94

  4. 3D Organoid and Organotypic Cultures

    1. Front Matter

      Pages 95-95
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    2. Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells

      • Shinya Sugimoto, Toshiro Sato
      Pages 97-105

    3. 3D Coculture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial–Stromal Interactions During Mammary Branching Morphogenesis

      • Zuzana Koledova
      Pages 107-124

    4. An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis

      • Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, Christina H. Scheel
      Pages 125-137

    5. 3D Primary Culture Model to Study Human Mammary Development

      • Daniel H. Miller, Ethan S. Sokol, Piyush B. Gupta
      Pages 139-147

    6. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

      • Anas Rabata, Ales Hampl, Zuzana Koledova
      Pages 149-165

    7. 3D Hanging Drop Culture to Establish Prostate Cancer Organoids

      • Theresa Eder, Iris E. Eder
      Pages 167-175

    8. 3D-Dynamic Culture Models of Multiple Myeloma

      • Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni et al.
      Pages 177-190

    9. Preparation of a Three-Dimensional Full Thickness Skin Equivalent

      • Christian Reuter, Heike Walles, Florian Groeber
      Pages 191-198

    10. Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System

      • Romana E. Ranftl, Fernando Calvo
      Pages 199-212Open Access

    11. 3D Coculture Model of the Brain Parenchyma–Metastasis Interface of Brain Metastasis

      • Raquel Blazquez, Tobias Pukrop
      Pages 213-222

  5. Micropatterning

    1. Front Matter

      Pages 223-223
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Keywords

About this book

This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures.




Chapter 15 of this book is available open access under a CC BY 4.0 license.

Editors and Affiliations

  • Department of Histology and Embryology, Masaryk University, Brno, Czech Republic

    Zuzana Koledova

Bibliographic Information

  • Book Title: 3D Cell Culture

  • Book Subtitle: Methods and Protocols

  • Editors: Zuzana Koledova

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7021-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7019-3Published: 21 June 2017

  • Softcover ISBN: 978-1-4939-8370-4Published: 12 June 2018

  • eBook ISBN: 978-1-4939-7021-6Published: 19 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 452

  • Number of Illustrations: 15 b/w illustrations, 99 illustrations in colour

  • Topics: Cell Biology

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