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Molecular Profiling

Methods and Protocols

  • Book
  • © 2017
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Overview

Editors:
  1. Virginia Espina

    1. Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1606)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Tumor Staging and Grading: A Primer

    • Stacy M. Telloni
    Pages 1-17
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  3. Innovations in Clinical Trial Design in the Era of Molecular Profiling

    • Julia D. Wulfkuhle, Alexander Spira, Kirsten H. Edmiston, Emanuel F. Petricoin III
    Pages 19-36
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  4. Personalized Medicine: Ethical Aspects

    • G. Terry Sharrer
    Pages 37-50
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  5. Antibody Validation by Western Blotting

    • Michele Signore, Valeria Manganelli, Alex Hodge
    Pages 51-70
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  6. Scanning Electron Microscopy Sample Preparation and Imaging

    • Jenny Ngoc Tran Nguyen, Amanda M. Harbison
    Pages 71-84
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  7. One-Step Preservation and Decalcification of Bony Tissue for Molecular Profiling

    • Claudius Mueller, Michael G. Harpole, Virginia Espina
    Pages 85-102
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  8. Application of Hydrogel Nanoparticles for the Capture, Concentration, and Preservation of Low-Abundance Biomarkers

    • Ruben Magni, Alessandra Luchini
    Pages 103-113
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  9. Using Laser Capture Microdissection to Isolate Cortical Laminae in Nonhuman Primate Brain

    • Brian A. Corgiat, Claudius Mueller
    Pages 115-132
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  10. Western Blot Techniques

    • Brianna Kim
    Pages 133-139
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  11. ELISA for Monitoring Nerve Growth Factor

    • Justin B. Davis
    Pages 141-147
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  12. Reverse Phase Protein Microarrays

    • Elisa Baldelli, Valerie Calvert, Alex Hodge, Amy VanMeter, Emanuel F. Petricoin III, Mariaelena Pierobon
    Pages 149-169
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  13. Clustering and Network Analysis of Reverse Phase Protein Array Data

    • Adam Byron
    Pages 171-191
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  14. PCR: Identification of Genetic Polymorphisms

    • Amanda M. Harbison, Jenny Ngoc Tran Nguyen
    Pages 193-203
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  15. Microsatellite Analysis for Identification of Individuals Using Bone from the Extinct Steller’s Sea Cow (Hydrodamalis gigas)

    • Jeffery F. Warner, Michael G. Harpole, Lorelei D. Crerar
    Pages 205-217
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  16. Somatic DNA Mutation Analysis

    • Anthony O’Grady, Robert Cummins
    Pages 219-233
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  17. Optimization of Immunostaining for Prospective Image Analysis

    • Scott M. Lawrence, Yelena G. Golubeva
    Pages 235-263
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  18. Fluorescence In Situ Hybridization of Cells, Chromosomes, and Formalin-Fixed Paraffin-Embedded Tissues

    • Ahmad Alamri, Jun Yeb Nam, Jan K. Blancato
    Pages 265-279
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  19. High-Resolution Image Stitching as a Tool to Assess Tissue-Level Protein Distribution and Localization

    • Bryan A. Millis, Matthew J. Tyska
    Pages 281-296
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  20. Mass Spectrometry-Based Biomarker Discovery

    • Weidong Zhou, Emanuel F. Petricoin III, Caterina Longo
    Pages 297-311
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Keywords

About this book

This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery to clinical trial validation and commercial public benefit.  Chapters aim to cover the full spectrum of molecular profiling from tumor staging and grading through biomarker discovery, to commercialization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Profiling: Methods and Protocols, Second Edition aims to be a useful guide on molecular profiling. 

Editors and Affiliations

  • Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, USA

    Virginia Espina

Bibliographic Information

  • Book Title: Molecular Profiling

  • Book Subtitle: Methods and Protocols

  • Editors: Virginia Espina

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6990-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6989-0Published: 14 May 2017

  • Softcover ISBN: 978-1-4939-8360-5Published: 09 June 2018

  • eBook ISBN: 978-1-4939-6990-6Published: 11 May 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 414

  • Number of Illustrations: 23 b/w illustrations, 45 illustrations in colour

  • Topics: Molecular Medicine

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