Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1603)
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Table of contents (16 protocols)
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Front Matter
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Back Matter
About this book
This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.
Editors and Affiliations
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National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland
Paula Meleady
Bibliographic Information
Book Title: Heterologous Protein Production in CHO Cells
Book Subtitle: Methods and Protocols
Editors: Paula Meleady
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6972-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6971-5Published: 11 May 2017
Softcover ISBN: 978-1-4939-8356-8Published: 20 July 2018
eBook ISBN: 978-1-4939-6972-2Published: 09 May 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 252
Number of Illustrations: 7 b/w illustrations, 28 illustrations in colour
Topics: Protein Science