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Heterologous Protein Production in CHO Cells

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Paula Meleady

    1. National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1603)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Strategies and Considerations for Improving Expression of “Difficult to Express” Proteins in CHO Cells

    • Christina S. Alves, Terrence M. Dobrowsky
    Pages 1-23

  3. Glycoengineering of CHO Cells to Improve Product Quality

    • Qiong Wang, Bojiao Yin, Cheng-Yu Chung, Michael J. Betenbaugh
    Pages 25-44

  4. Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension

    • Yashas Rajendra, Sowmya Balasubramanian, David L. Hacker
    Pages 45-55

  5. Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression

    • Patrick Mayrhofer, Renate Kunert
    Pages 57-69

  6. Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells

    • Eric Baek, Soo Min Noh, Gyun Min Lee
    Pages 71-85

  7. Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-SanDI Sponge Vectors

    • Alan Costello, Nga Lao, Martin Clynes, Niall Barron
    Pages 87-100

  8. Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells

    • Lise Marie Grav, Karen Julie la Cour Karottki, Jae Seong Lee, Helene Faustrup Kildegaard
    Pages 101-118

  9. Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture

    • Zeynep Betts, Alan J. Dickson
    Pages 119-141

  10. Selection of High-Producing Clones Using FACS for CHO Cell Line Development

    • Clair Gallagher, Paul S. Kelly
    Pages 143-152

  11. The ‘Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity

    • Hussain Dahodwala, Susan T. Sharfstein
    Pages 153-168

  12. A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data

    • Craig Monger, Krishna Motheramgari, John McSharry, Niall Barron, Colin Clarke
    Pages 169-186

  13. Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells

    • Orla Coleman, Michael Henry, Martin Clynes, Paula Meleady
    Pages 187-194

  14. Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells

    • Michael Henry, Orla Coleman, Prashant, Martin Clynes, Paula Meleady
    Pages 195-208

  15. Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture

    • Yuzhou Fan, Helene Faustrup Kildegaard, Mikael Rørdam Andersen
    Pages 209-226

  16. Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells

    • Sara Carillo, Stefan Mittermayr, Amy Farrell, Simone Albrecht, Jonathan Bones
    Pages 227-241

  17. Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses

    • Catherine E. M. Hogwood, Lesley M. Chiverton, C. Mark Smales
    Pages 243-250

  18. Back Matter

    Pages 251-252
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Keywords

About this book

This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic approaches. This book also explores methods that discuss the genome editing tool, CRISPR/Cas9, and the characterization of recombinant protein products, such as glycosylation and host cell protein analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Heterologous Protein Production in CHO Cells: Methods and Protocols is a valuable resource for scientists and researchers who are interested in further studying cell production in CHO cells.

Editors and Affiliations

  • National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland

    Paula Meleady

Bibliographic Information

  • Book Title: Heterologous Protein Production in CHO Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Paula Meleady

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6972-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6971-5Published: 11 May 2017

  • Softcover ISBN: 978-1-4939-8356-8Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6972-2Published: 09 May 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 252

  • Number of Illustrations: 7 b/w illustrations, 28 illustrations in colour

  • Topics: Protein Science

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