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  • © 2017

Cell Viability Assays

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1601)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
  2. Basic Colorimetric Proliferation Assays: MTT, WST, and Resazurin

    • Konstantin Präbst, Hannes Engelhardt, Stefan Ringgeler, Holger Hübner
    Pages 1-17
  3. Assaying Cellular Viability Using the Neutral Red Uptake Assay

    • Gamze Ates, Tamara Vanhaecke, Vera Rogiers, Robim M. Rodrigues
    Pages 19-26
  4. Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image Cytometry

    • Leo Li-Ying Chan, Kelsey J. McCulley, Sarah L. Kessel
    Pages 27-41
  5. High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity

    • Delyan P. Ivanov, Anna M. Grabowska, Martin C. Garnett
    Pages 43-59
  6. Ferroptosis and Cell Death Analysis by Flow Cytometry

    • Daishi Chen, Ilker Y. Eyupoglu, Nicolai Savaskan
    Pages 71-77
  7. Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness

    • Natalia Smolina, Joseph Bruton, Anna Kostareva, Thomas Sejersen
    Pages 79-87
  8. An ATP-Based Luciferase Viability Assay for Animal African Trypanosomes Using a 96-Well Plate

    • Keisuke Suganuma, Nthatisi Innocentia Molefe, Noboru Inoue
    Pages 89-95
  9. SYBR® Green I-Based Fluorescence Assay to Assess Cell Viability of Malaria Parasites for Routine Use in Compound Screening

    • Maria Leidenberger, Cornelia Voigtländer, Nina Simon, Barbara Kappes
    Pages 97-110
  10. Screening Applications to Test Cellular Fitness in Transwell® Models After Nanoparticle Treatment

    • Bastian Christ, Christina Fey, Alevtina Cubukova, Heike Walles, Sofia Dembski, Marco Metzger
    Pages 111-122
  11. Metabolite Profiling of Mammalian Cell Culture Processes to Evaluate Cellular Viability

    • Isobelle M. Evie, Alan J. Dickson, Mark Elvin
    Pages 137-152
  12. Assaying Spontaneous Network Activity and Cellular Viability Using Multi-well Microelectrode Arrays

    • Jasmine P. Brown, Brittany S. Lynch, Itaevia M. Curry-Chisolm, Timothy J. Shafer, Jenna D. Strickland
    Pages 153-170
  13. Quantitative Ratiometric Ca2+ Imaging to Assess Cell Viability

    • Oliver Friedrich, Stewart I. Head
    Pages 171-193
  14. Functional Viability: Measurement of Synaptic Vesicle Pool Sizes

    • Jana K. Wrosch, Teja W. Groemer
    Pages 195-204
  15. Phenotyping Cellular Viability by Functional Analysis of Ion Channels: GlyR-Targeted Screening in NT2-N Cells

    • Katharina Kuenzel, Sepideh Abolpour Mofrad, Daniel F. Gilbert
    Pages 205-214
  16. Systematic Cell-Based Phenotyping of Missense Alleles

    • Aenne S. Thormählen, Heiko Runz
    Pages 215-228

About this book

This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cell Viability Assays: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Friedrich-Alexander University (FAU) Erlangen-Nürnberg, Institute of Medical Biotechnology, Erlangen, Germany

    Daniel F. Gilbert, Oliver Friedrich

About the editors





Bibliographic Information

  • Book Title: Cell Viability Assays

  • Book Subtitle: Methods and Protocols

  • Editors: Daniel F. Gilbert, Oliver Friedrich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6960-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6959-3Published: 03 May 2017

  • Softcover ISBN: 978-1-4939-8352-0Published: 09 June 2018

  • eBook ISBN: 978-1-4939-6960-9Published: 03 May 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 299

  • Number of Illustrations: 20 b/w illustrations, 50 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access