Neuroproteomics

1. Front Matter

   Pages i-xx

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2. Current Reviews of Neuroproteomics Approaches and Applications

   1. Front Matter

      Pages 1-1

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   2. Neuroproteomics Studies: Challenges and Updates

      • Naify Ramadan, Hussein Ghazale, Mohammad El-Sayyad, Mohamad El-Haress, Firas H. Kobeissy

      Pages 3-19

   3. Progress and Potential of Imaging Mass Spectrometry Applied to Biomarker Discovery

      • Jusal Quanico, Julien Franck, Maxence Wisztorski, Michel Salzet, Isabelle Fournier

      Pages 21-43

   4. Biofluid Proteomics and Biomarkers in Traumatic Brain Injury

      • Safa Azar, Anwarul Hasan, Richard Younes, Farah Najdi, Lama Baki, Hussein Ghazale et al.

      Pages 45-63

   5. Degradomics in Neurotrauma: Profiling Traumatic Brain Injury

      • Hadi Abou-El-Hassan, Fares Sukhon, Edwyn Jeremy Assaf, Hisham Bahmad, Hussein Abou-Abbass, Hussam Jourdi et al.

      Pages 65-99

   6. Evolving Relevance of Neuroproteomics in Alzheimer’s Disease

      • Simone Lista, Henrik Zetterberg, Sid E. O’Bryant, Kaj Blennow, Harald Hampel

      Pages 101-115

   7. Genome to Phenome: A Systems Biology Approach to PTSD Using an Animal Model

      • Nabarun Chakraborty, James Meyerhoff, Marti Jett, Rasha Hammamieh

      Pages 117-154

3. Experimental Methods

   1. Front Matter

      Pages 155-155

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   2. Photoaffinity Labeling of Pentameric Ligand-Gated Ion Channels: A Proteomic Approach to Identify Allosteric Modulator Binding Sites

      • Selwyn S. Jayakar, Gordon Ang, David C. Chiara, Ayman K. Hamouda

      Pages 157-197

   3. Quantitative Phosphoproteomic Analysis of Brain Tissues

      • Bing Bai, Haiyan Tan, Junmin Peng

      Pages 199-211

   4. Glycoproteins Enrichment and LC-MS/MS Glycoproteomics in Central Nervous System Applications

      • Rui Zhu, Ehwang Song, Ahmed Hussein, Firas H. Kobeissy, Yehia Mechref

      Pages 213-227

   5. A Novel 2-DE-Based Proteomic Analysis to Identify Multiple Substrates for Specific Protease in Neuronal Cells

      • Chiho Kim, Young J. Oh

      Pages 229-245

   6. Neuroproteomic Profiling of Cerebrospinal Fluid (CSF) by Multiplexed Affinity Arrays

      • Anna Häggmark-Månberg, Peter Nilsson, Jochen M. Schwenk

      Pages 247-254

   7. Isolation and Proteomic Analysis of Microvesicles and Exosomes from HT22 Cells and Primary Neurons

      • Richard Witas, Dale Chaput, Hirah Khan, Stanley M. Stevens Jr., David Kang

      Pages 255-267

   8. Combined MALDI Mass Spectrometry Imaging and Parafilm-Assisted Microdissection-Based LC-MS/MS Workflows in the Study of the Brain

      • Jusal Quanico, Julien Franck, Maxence Wisztorski, Michel Salzet, Isabelle Fournier

      Pages 269-283

   9. De Novo and Uninterrupted SILAC Labeling of Primary Microglia

      • Ping Zhang, Ashley Culver-Cochran, Stanley M. Stevens Jr., Bin Liu

      Pages 285-293

   10. Spike-In SILAC Approach for Proteomic Analysis of Ex Vivo Microglia

       • Joao Paulo Costa Pinho, Harris Bell-Temin, Bin Liu, Stanley M. Stevens Jr.

       Pages 295-312

   11. A Proteomic Evaluation of Sympathetic Activity Biomarkers of the Hypothalamus-Pituitary-Adrenal Axis by Western Blotting Technique Following Experimental Traumatic Brain Injury

       • Hale Zerrin Toklu, Yasemin Sakarya, Nihal Tümer

       Pages 313-325

4. Bioinformatic and Computational Methods

   1. Front Matter

      Pages 327-327

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This new edition provides updated and novel protocols of neuroproteomics methods that encompass both global-scale as well as targeted and specialized topics, which are timely additions for the molecular and phenotypic analysis of the central nervous system and CNS-related disorders. The detailed contents of this book include the exploration of several exciting areas of advanced methods used for neuroproteomics research including relative and absolute protein quantitation by mass spectrometry, characterization of post-translational modifications, as well as bioinformatics and computational approaches. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Updated and accessible, Neuroproteomics: Methods and Protocols, Second Editionserves researchers and clinical scientists involved in the area of biomarker research and protein biochemistry, as well as molecular biologists and biochemists who have been involved in proteomics research already or even for those new to the field.

• Central nervous system
• Neurobiology
• Protein quantitation
• Post-translational modifications
• Bioinformatics
• Mass spectrometry

• Department of Psychiatry, University of Florida McKnight Brain Institute, Gainesville, USA

  Firas H. Kobeissy

• Department of Cell Biology, Microbiology, & Molecular Biology, University of South Florida, Tampa, USA

  Stanley M. Stevens, Jr.

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