Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1593)
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Table of contents (25 protocols)
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Front Matter
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Flagellar Type III Protein Export, Assembly and Gene Regulation in Salmonella enterica
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Front Matter
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Structure of the Bacterial Flagellum
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Front Matter
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Dynamics of the Bacterial Flagellar Motor
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Front Matter
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Keywords
About this book
This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.
Editors and Affiliations
Bibliographic Information
Book Title: The Bacterial Flagellum
Book Subtitle: Methods and Protocols
Editors: Tohru Minamino, Keiichi Namba
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6927-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6926-5Published: 08 April 2017
Softcover ISBN: 978-1-4939-8341-4Published: 21 June 2018
eBook ISBN: 978-1-4939-6927-2Published: 07 April 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 326
Number of Illustrations: 35 b/w illustrations, 44 illustrations in colour
Topics: Microbiology