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Stem Cell Banking

Concepts and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Jeremy M. Crook

    1. ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Squires Way, Fairy Meadow, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Tenneille E. Ludwig

    1. WiCell Research Institute, Madison, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1590)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Generic Themes in Stem Cell Banking

    1. Front Matter

      Pages 1-1
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    2. Stem Cell Banking: A Global View

      • Glyn Stacey
      Pages 3-10

    3. Quality Assurance in Stem Cell Banking: Emphasis on Embryonic and Induced Pluripotent Stem Cell Banking

      • Therése Kallur, Pontus Blomberg, Sonya Stenfelt, Kristian Tryggvason, Outi Hovatta
      Pages 11-16

    4. Acquisition and Reception of Primary Tissues, Cells, or Other Biological Specimens

      • Lyn E. Healy
      Pages 17-27

    5. Information Management

      • Alberto Labarga, Izaskun Beloqui, Angel G. Martin
      Pages 29-39

    6. Cryopreservation: Vitrification and Controlled Rate Cooling

      • Charles J. Hunt
      Pages 41-77

    7. Quality Assured Characterization of Stem Cells for Safety in Banking for Clinical Application

      • Kevin W. Bruce, John D. M. Campbell, Paul De Sousa
      Pages 79-98

    8. Ethics and Governance of Stem Cell Banks

      • Donald Chalmers, Peter Rathjen, Joy Rathjen, Dianne Nicol
      Pages 99-112

  3. Protocols for Pluripotent Stem Cell Banking

    1. Front Matter

      Pages 113-113
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    2. Derivation of Human Embryonic Stem Cells

      • Jeremy M. Crook, Lucy Kravets, Teija Peura, Meri T. Firpo
      Pages 115-129

    3. Derivation of Human-Induced Pluripotent Stem Cells in Chemically Defined Medium

      • Guokai Chen, Mahendra Rao
      Pages 131-137

    4. Culture, Adaptation, and Expansion of Pluripotent Stem Cells

      • Jennifer L. Brehm, Tenneille E. Ludwig
      Pages 139-150

    5. Cryobanking Pluripotent Stem Cells

      • Jeremy M. Crook, Eva Tomaskovic-Crook, Tenneille E. Ludwig
      Pages 151-164

    6. Genome Editing in Human Pluripotent Stem Cells

      • Jared Carlson-Stevermer, Krishanu Saha
      Pages 165-174

  4. Protocols for Mesenchymal Stem Cell Banking

    1. Front Matter

      Pages 175-175
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    2. Isolation, Culture, and Expansion of Mesenchymal Stem Cells

      • Izaskun Ferrin, Izaskun Beloqui, Lorea Zabaleta, Juan M. Salcedo, Cesar Trigueros, Angel G. Martin
      Pages 177-190

    3. Cryobanking Mesenchymal Stem Cells

      • Andrés Pavón, Izaskun Beloqui, Juan M. Salcedo, Angel G. Martin
      Pages 191-196

  5. Protocols for Human Neural Stem Cell Banking

    1. Front Matter

      Pages 197-197
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    2. Culturing and Cryobanking Human Neural Stem Cells

      • Jeremy M. Crook, Eva Tomaskovic-Crook
      Pages 199-206
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Keywords

About this book

This volume covers protocols related to both pluripotent and somatic stem cells, including the ethical procurement of tissues and cells for the provision of “seed stock,” standardized methods for deriving hESCs and iPSCs, isolating mesenchymal stem cells, cell culture and cryopreservation, in addition to quality assurance and information management. Stem Cell Banking: Concepts and Protocols aims to contribute to the development of this field by providing information that is essential to establishing a bona fide stem cell bank. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Stem Cell Banking: Concepts and Protocols is a valuable resource for stem cell scientists and novices to the field, and will help strengthen and maximize their use of existing and future stem cell resources.

 

Editors and Affiliations

  • ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Squires Way, Fairy Meadow, Australia

    Jeremy M. Crook

  • WiCell Research Institute, Madison, USA

    Tenneille E. Ludwig

Bibliographic Information

  • Book Title: Stem Cell Banking

  • Book Subtitle: Concepts and Protocols

  • Editors: Jeremy M. Crook, Tenneille E. Ludwig

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6921-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6919-7Published: 29 March 2017

  • Softcover ISBN: 978-1-4939-8339-1Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6921-0Published: 28 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 210

  • Number of Illustrations: 8 b/w illustrations, 10 illustrations in colour

  • Topics: Cell Biology

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