Editors:
- Includes cutting-edge methods and protocols in the MiMB standard format
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1590)
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Table of contents (15 protocols)
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Front Matter
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Generic Themes in Stem Cell Banking
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Front Matter
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Protocols for Pluripotent Stem Cell Banking
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Front Matter
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Protocols for Mesenchymal Stem Cell Banking
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Front Matter
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Protocols for Human Neural Stem Cell Banking
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Front Matter
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About this book
This volume covers protocols related to both pluripotent and somatic stem cells, including the ethical procurement of tissues and cells for the provision of “seed stock,” standardized methods for deriving hESCs and iPSCs, isolating mesenchymal stem cells, cell culture and cryopreservation, in addition to quality assurance and information management. Stem Cell Banking: Concepts and Protocols aims to contribute to the development of this field by providing information that is essential to establishing a bona fide stem cell bank. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Stem Cell Banking: Concepts and Protocols is a valuable resource for stem cell scientists and novices to the field, and will help strengthen and maximize their use of existing and future stem cell resources.
Editors and Affiliations
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ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Squires Way, Fairy Meadow, Australia
Jeremy M. Crook
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WiCell Research Institute, Madison, USA
Tenneille E. Ludwig
Bibliographic Information
Book Title: Stem Cell Banking
Book Subtitle: Concepts and Protocols
Editors: Jeremy M. Crook, Tenneille E. Ludwig
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6921-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6919-7Published: 29 March 2017
Softcover ISBN: 978-1-4939-8339-1Published: 20 July 2018
eBook ISBN: 978-1-4939-6921-0Published: 28 March 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 210
Number of Illustrations: 8 b/w illustrations, 10 illustrations in colour
Topics: Cell Biology