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Telomeres and Telomerase

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Zhou Songyang

    1. Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1587)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to Telomeres and Telomerase

    • Zhou Songyang
    Pages 1-13

  3. Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9

    • Jun Xu, Zhou Songyang, Dan Liu, Hyeung Kim
    Pages 15-28

  4. Telomere Length Analysis by Quantitative Fluorescent in Situ Hybridization (Q-FISH)

    • Isabelle Ourliac-Garnier, Arturo Londoño-Vallejo
    Pages 29-39

  5. Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH)

    • Isabelle Ourliac-Garnier, Arturo Londoño-Vallejo
    Pages 41-54

  6. Telomere G-Rich Overhang Length Measurement: DSN Method

    • Yong Zhao, Jerry W. Shay, Woodring E. Wright
    Pages 55-62

  7. Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA

    • Hidetoshi Tahara
    Pages 63-69

  8. Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In

    • Yong Zhao, Jerry W. Shay, Woodring E. Wright
    Pages 71-82

  9. Analysis of Yeast Telomerase by Primer Extension Assays

    • Min Hsu, Neal F. Lue
    Pages 83-93

  10. Assessing Telomerase Activities in Mammalian Cells Using the Quantitative PCR-Based Telomeric Repeat Amplification Protocol (qTRAP)

    • Shuai Jiang, Mengfan Tang, Huawei Xin, Junjiu Huang
    Pages 95-101

  11. Telomeres and NextGen CO-FISH: Directional Genomic Hybridization (Telo-dGH™)

    • Miles J. McKenna, Erin Robinson, Edwin H. Goodwin, Michael N. Cornforth, Susan M. Bailey
    Pages 103-112

  12. Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques

    • Eladio Abreu, Rebecca M. Terns, Michael P. Terns
    Pages 113-125

  13. Cytogenetic Analysis of Telomere Dysfunction

    • Rekha Rai, Asha S. Multani, Sandy Chang
    Pages 127-131

  14. Probing the Telomere Damage Response

    • Rekha Rai, Sandy Chang
    Pages 133-138

  15. Induction of Site-Specific Oxidative Damage at Telomeres by Killerred-Fused Shelretin Proteins

    • Rong Tan, Li Lan
    Pages 139-146

  16. Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein-Protein Interactions

    • Wenbin Ma, Ok-hee Lee, Hyeung Kim, Zhou Songyang
    Pages 147-160

  17. In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits

    • Jing Huang, Christopher J. Bley, Dustin P. Rand, Julian J. L. Chen, Ming Lei
    Pages 161-169

  18. Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds

    • Clement Lin, Danzhou Yang
    Pages 171-196

  19. Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization

    • Zepeng Zhang, Qian Hu, Yong Zhao
    Pages 197-204

  20. Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)

    • Feng Liu, Xuyang Feng, Wenbin Ma
    Pages 205-214
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Keywords

About this book

This third edition volume expands on the previous editions with new and improved assays that ask essential questions of telomere regulation in diverse model systems. The chapters in this book discuss genetic, proteomic, genomic, biochemical, and molecular approaches that provide insight into the complex protein interaction networks active in the telomere chromatin. This book also details information regarding telomere dynamics in response to stress or stimuli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Telomeres and Telomerase: Methods and Protocols, Third Edition is a valuable and informative resource for anyone who is interested in the developing field of telomeres and telomerase.

Editors and Affiliations

  • Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, USA

    Zhou Songyang

Bibliographic Information

  • Book Title: Telomeres and Telomerase

  • Book Subtitle: Methods and Protocols

  • Editors: Zhou Songyang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6892-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6891-6Published: 21 March 2017

  • Softcover ISBN: 978-1-4939-8330-8Published: 09 June 2018

  • eBook ISBN: 978-1-4939-6892-3Published: 21 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 218

  • Number of Illustrations: 19 b/w illustrations, 18 illustrations in colour

  • Topics: Biochemistry, general

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