Skip to main content
SpringerLink
Log in
Book cover

Techniques to Investigate Mitochondrial Function in Neurons

  • Book
  • © 2017

Overview

Editors:
  1. Stefan Strack

    1. Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Yuriy M. Usachev

    1. Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Neuromethods (NM, volume 123)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Three-Dimensional Reconstruction of Neuronal Mitochondria by Electron Tomography

    • Guy Perkins
    Pages 1-29

  3. Measuring Mitochondrial Shape with ImageJ

    • Ronald A. Merrill, Kyle H. Flippo, Stefan Strack
    Pages 31-48

  4. Live Imaging Mitochondrial Transport in Neurons

    • Meredith M. Course, Chung-Han Hsieh, Pei-I Tsai, Jennifer A. Codding-Bui, Atossa Shaltouki, Xinnan Wang
    Pages 49-66

  5. Techniques to Investigate Bioenergetics of Mitochondria

    • William I. Sivitz
    Pages 67-94

  6. Respirometry in Neurons

    • Liang Zhang, Eugenia Trushina
    Pages 95-113

  7. Measuring ATP in Axons with FRET

    • Lauren Y. Shields, Bryce A. Mendelsohn, Ken Nakamura
    Pages 115-131

  8. Characterizing Metabolic States Using Fluorescence Lifetime Imaging Microscopy (FLIM) of NAD(P)H

    • Thomas S. Blacker, Michael R. Duchen
    Pages 133-150

  9. Techniques for Simultaneous Mitochondrial and Cytosolic Ca2+ Imaging in Neurons

    • Jacob E. Rysted, Zhihong Lin, Yuriy M. Usachev
    Pages 151-178

  10. Live Imaging of Mitochondrial ROS Production and Dynamic Redox Balance in Neurons

    • Karolina Can, Sebastian Kügler, Michael Müller
    Pages 179-197

  11. Monitoring of Permeability Transition Pore Openings in Isolated Individual Brain Mitochondria

    • Nickolay Brustovetsky, Tatiana Brustovetsky
    Pages 199-210

  12. Examination of Mitochondrial Ion Conductance by Patch Clamp in Intact Neurons and Mitochondrial Membrane Preparations

    • Elizabeth A. Jonas, Nelli Mnatsakanyan
    Pages 211-238

  13. Monitoring of Permeability Transition Pore Openings in Mitochondria of Cultured Neurons

    • Tatiana Brustovetsky, Nickolay Brustovetsky
    Pages 239-248

  14. Protocols for Assessing Mitophagy in Neuronal Cell Lines and Primary Neurons

    • Ruben K. Dagda, Monica Rice
    Pages 249-277

  15. Examining Mitochondrial Function at Synapses In Situ

    • Gregory T. Macleod, Maxim V. Ivannikov
    Pages 279-297

  16. Proteomic Analysis of Neuronal Mitochondria

    • Kelly L. Stauch, Howard S. Fox
    Pages 299-319

  17. Measuring Mitochondrial Pyruvate Oxidation

    • Lawrence R. Gray, Alix A. J. Rouault, Lalita Oonthonpan, Adam J. Rauckhorst, Julien A. Sebag, Eric B. Taylor
    Pages 321-338

  18. Back Matter

    Pages 339-344
    Download chapter PDF
Back to top

Keywords

About this book

This volume describes a broad spectrum of experimental approaches for investigating structure, function, and transport of neuronal mitochondria in health and disease. Most of these approaches were only recently developed and range from electron tomography-based 3D reconstruction of mitochondrial cristae to patch clamp recording from mitochondria in intact neurons. The chapters in this book cover topics such as mitochondrial proteomics, fluorescence lifetime imaging, respirometry and mitophagy, as well as optical approaches based on the use of genetically engineered fluorescent sensors for monitoring synaptic ATP and axonal ROS generation, mitochondrial Ca2+ cycling and pH changes, and mitochondrial dynamics and axonal trafficking in live neurons. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 
Cutting-edge and comprehensive, Techniques to Investigate Mitochondrial Function in Neurons is a valuable and useful resource for a broad range of investigators interested in the function of neuronal mitochondria in health and disease states.

 

Editors and Affiliations

  • Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, USA

    Stefan Strack, Yuriy M. Usachev

Bibliographic Information

  • Book Title: Techniques to Investigate Mitochondrial Function in Neurons

  • Editors: Stefan Strack, Yuriy M. Usachev

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-6890-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6888-6Published: 18 March 2017

  • Softcover ISBN: 978-1-4939-8329-2Published: 07 August 2018

  • eBook ISBN: 978-1-4939-6890-9Published: 16 March 2017

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XV, 344

  • Number of Illustrations: 23 b/w illustrations, 51 illustrations in colour

  • Topics: Neurosciences

Publish with us

Policies and ethics

Back to top

Search

Navigation