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Human T-Lymphotropic Viruses

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Claudio Casoli

    1. University of Milano, Milano, Italy

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  • Includes cutting-edge methods and protocols for the study of HTLVs
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1582)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Epidemiology and Transmission

    1. Front Matter

      Pages 1-1
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    2. Serological and Molecular Methods to Study Epidemiological Aspects of Human T-Cell Lymphotropic Virus Type 1 Infection

      • Olivier Cassar, Antoine Gessain
      Pages 3-24

    3. Molecular Epidemiology Database for Sequence Management and Data Mining

      • Thessika Hialla Almeida Araújo, Filipe Ferreira de Almeida Rego, Luiz Carlos Junior Alcantara
      Pages 25-31

    4. Reporter Systems to Study HTLV-1 Transmission

      • Christine Gross, Andrea K. Thoma-Kress
      Pages 33-46

    5. Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Infection Using Co-Culture with Jurkat LTR-Luciferase or Jurkat LTR-GFP Reporter Cells

      • Sandrine Alais, Hélène Dutartre, Renaud Mahieux
      Pages 47-55

    6. Isolation of Exosomes from HTLV-Infected Cells

      • Robert A. Barclay, Michelle L. Pleet, Yao Akpamagbo, Kinza Noor, Allison Mathiesen, Fatah Kashanchi
      Pages 57-75

  3. Promoter Activity of HTLV Proteins

    1. Front Matter

      Pages 77-77
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    2. A Luciferase Functional Quantitative Assay for Measuring NF-ĸB Promoter Transactivation Mediated by HTLV-1 and HTLV-2 Tax Proteins

      • Elisa Bergamo, Erica Diani, Umberto Bertazzoni, Maria Grazia Romanelli
      Pages 79-87

    3. Generation of a Tet-On Expression System to Study Transactivation Ability of Tax-2

      • Fabio Bignami, Riccardo Alessio Sozzi, Elisabetta Pilotti
      Pages 89-101

    4. EGF Uptake and Degradation Assay to Determine the Effect of HTLV Regulatory Proteins on the ESCRT-Dependent MVB Pathway

      • Colin Murphy, Noreen Sheehy
      Pages 103-108

  4. Genotyping and Gene Expression

    1. Front Matter

      Pages 109-109
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    2. Methods for Identifying and Examining HTLV-1 HBZ Post-translational Modifications

      • Jacob Al-Saleem, Mamuka Kvaratskhelia, Patrick L. Green
      Pages 111-126

    3. High-Throughput Mapping and Clonal Quantification of Retroviral Integration Sites

      • Nicolas A. Gillet, Anat Melamed, Charles R. M. Bangham
      Pages 127-141

    4. STR Profiling of HTLV-1-Infected Cell Lines

      • Vittoria Raimondi, Sonia Minuzzo, Vincenzo Ciminale, Donna M. D’Agostino
      Pages 143-154

    5. Expression of HTLV-1 Genes in T-Cells Using RNA Electroporation

      • Mariangela Manicone, Francesca Rende, Ilaria Cavallari, Andrea K. Thoma-Kress, Vincenzo Ciminale
      Pages 155-170

  5. Cellular Dynamics

    1. Front Matter

      Pages 171-171
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    2. Quantification of Cell Turnover in the Bovine Leukemia Virus Model

      • Alix de Brogniez, Pierre-Yves Barez, Alexandre Carpentier, Geronimo Gutierrez, Michal Reichert, Karina Trono et al.
      Pages 173-182

    3. Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection

      • Federica Bozzano, Francesco Marras, Andrea De Maria
      Pages 183-194

  6. Therapy

    1. Front Matter

      Pages 195-195
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Keywords

About this book

While HIV-1 continues to be well-researched, this detailed volume draws attention to other members of the Retrovirus family, namely the Human T-lymphotropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination and the methods to investigate their interaction with the host immune system and interfering pathogens. The contents include essential aspects of epidemiology and virus transmission, novel and robust methodologies for studying the effects of trans-activating regulatory HTLVs’ proteins, the latest techniques for genotyping and gene expression analysis, as well as cellular phenotype and dynamics.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Human T-Lymphotropic Viruses: Methods and Protocols serves as an ideal guide to an area of study that is very much worthy of further research.

Editors and Affiliations

  • University of Milano, Milano, Italy

    Claudio Casoli

Bibliographic Information

  • Book Title: Human T-Lymphotropic Viruses

  • Book Subtitle: Methods and Protocols

  • Editors: Claudio Casoli

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6872-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-6870-1Published: 30 March 2017

  • Softcover ISBN: 978-1-4939-8324-7Published: 07 August 2018

  • eBook ISBN: 978-1-4939-6872-5Published: 29 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 220

  • Number of Illustrations: 28 b/w illustrations, 15 illustrations in colour

  • Topics: Infectious Diseases

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