Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1579)
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Table of contents (17 protocols)
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Front Matter
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Expression and Purification of Matrix Metalloproteases
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Front Matter
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Structural Characterization of Matrix Metalloproteases
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Front Matter
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Computational Simulations of Matrix Metalloproteases
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Front Matter
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Determining Matrix Metalloprotease Substrate Specificity
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Front Matter
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Detection of Matrix Metalloproteases
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Front Matter
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Matrix Metalloprotease Inhibitors
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Front Matter
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About this book
Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Editors and Affiliations
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Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Australia
Charles A. Galea
Bibliographic Information
Book Title: Matrix Metalloproteases
Book Subtitle: Methods and Protocols
Editors: Charles A. Galea
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6863-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6861-9Published: 16 March 2017
Softcover ISBN: 978-1-4939-8321-6Published: 07 August 2018
eBook ISBN: 978-1-4939-6863-3Published: 15 March 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 314
Number of Illustrations: 22 b/w illustrations, 34 illustrations in colour
Topics: Biochemistry, general