Skip to main content
SpringerLink
Log in
Book cover

Matrix Metalloproteases

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Charles A. Galea

    1. Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1579)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Expression and Purification of Matrix Metalloproteases

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Expression and Purification of Matrix Metalloproteinases in Escherichia coli

      • Krishna K. Singh, Ruchi Jain, Harini Ramanan, Deepak K. Saini
      Pages 3-16

    3. Expression and Purification of a Matrix Metalloprotease Transmembrane Domain in Escherichia coli

      • Charles A. Galea
      Pages 17-33

    4. Heterologous Expression of the Astacin Protease Meprin β in Pichia pastoris

      • Dagmar Schlenzig, Stephan Schilling
      Pages 35-45

  3. Structural Characterization of Matrix Metalloproteases

    1. Front Matter

      Pages 47-47
      Download chapter PDF

    2. Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction

      • Elena Decaneto, Wolfgang Lubitz, Hideaki Ogata
      Pages 49-60

    3. Mapping Lipid Bilayer Recognition Sites of Metalloproteinases and Other Prospective Peripheral Membrane Proteins

      • Tara C. Marcink, Rama K. Koppisetti, Yan G. Fulcher, Steven R. Van Doren
      Pages 61-86

    4. Using Small Angle X-Ray Scattering (SAXS) to Characterize the Solution Conformation and Flexibility of Matrix Metalloproteinases (MMPs)

      • Louise E. Butt, Robert A. Holland, Nikul S. Khunti, Debra L. Quinn, Andrew R. Pickford
      Pages 87-108

  4. Computational Simulations of Matrix Metalloproteases

    1. Front Matter

      Pages 109-109
      Download chapter PDF

    2. Molecular Dynamics Studies of Matrix Metalloproteases

      • Natalia Díaz, Dimas Suárez
      Pages 111-134

  5. Determining Matrix Metalloprotease Substrate Specificity

    1. Front Matter

      Pages 135-135
      Download chapter PDF

    2. Determining the Substrate Specificity of Matrix Metalloproteases using Fluorogenic Peptide Substrates

      • Maciej J. Stawikowski, Anna M. Knapinska, Gregg B. Fields
      Pages 137-183

    3. Time-Resolved Analysis of Matrix Metalloproteinase Substrates in Complex Samples

      • Pascal Schlage, Fabian E. Egli, Ulrich auf dem Keller
      Pages 185-198

    4. Identification of Protease Cleavage Sites by Charge-Based Enrichment of Protein N-Termini

      • Zon W. Lai, Oliver Schilling
      Pages 199-207

    5. Mapping the Substrate Recognition Landscapes of Metalloproteases Using Comprehensive Mutagenesis

      • Colin A. Kretz
      Pages 209-228

  6. Detection of Matrix Metalloproteases

    1. Front Matter

      Pages 229-229
      Download chapter PDF

    2. Detection of Matrix Metalloproteinases by Zymography

      • Rajeev B. Tajhya, Rutvik S. Patel, Christine Beeton
      Pages 231-244

    3. Imaging Matrix Metalloproteases in Spontaneous Colon Tumors: Validation by Correlation with Histopathology

      • Harvey Hensley, Harry S. Cooper, Wen-Chi L. Chang, Margie L. Clapper
      Pages 245-255

  7. Matrix Metalloprotease Inhibitors

    1. Front Matter

      Pages 257-257
      Download chapter PDF
Back to top

Keywords

About this book

Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Australia

    Charles A. Galea

Bibliographic Information

  • Book Title: Matrix Metalloproteases

  • Book Subtitle: Methods and Protocols

  • Editors: Charles A. Galea

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6863-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6861-9Published: 16 March 2017

  • Softcover ISBN: 978-1-4939-8321-6Published: 07 August 2018

  • eBook ISBN: 978-1-4939-6863-3Published: 15 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 314

  • Number of Illustrations: 22 b/w illustrations, 34 illustrations in colour

  • Topics: Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation