Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1573)
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Table of contents (19 protocols)
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Front Matter
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Analysis of Ethylene Biosynthesis
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Front Matter
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Analysis of the Ethylene Signaling Pathway
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Front Matter
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Analysis of Ethylene Responses
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Front Matter
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Keywords
About this book
This volume provides a collection of protocols aimed toward the study of ethylene signaling in plants. Ethylene Signaling: Methods and Protocols is divided into three sections: ethylene biosynthesis, the signal transduction pathway, and the diverse ethylene responses of dicots and monocots. The chapters in section one discuss techniques for the measurement of activities related to the biosynthetic enzymes ACC synthase and ACC oxidase, the levels of ethylene synthesized by plants, and the treatment of plants with exogenous ethylene. Section two focuses on the analysis of the new membrane-associated proteins involved in the initial perception and transduction of the ethylene signal, such as ethylene receptors, CTR1, and EIN2. The third section covers assays applicable to dicots and monocots, including methods related to the roles of ethylene in germination, growth, abscission, abiotic stress, and defense. Section three also includes information on Arabidopsis mutantsand the variety of chemical inhibitors that affect ethylene responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and comprehensive, Ethylene Signaling: Methods and Protocols is a valuable resource for both experienced and beginner researchers with prior experience in the study of ethylene signaling and for those who are just entering this exciting research field.
Editors and Affiliations
Bibliographic Information
Book Title: Ethylene Signaling
Book Subtitle: Methods and Protocols
Editors: Brad M. Binder, G. Eric Schaller
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6854-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6852-7Published: 15 March 2017
Softcover ISBN: 978-1-4939-8318-6Published: 20 July 2018
eBook ISBN: 978-1-4939-6854-1Published: 13 March 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 272
Number of Illustrations: 22 b/w illustrations, 16 illustrations in colour
Topics: Plant Sciences