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Protein Terminal Profiling

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Oliver Schilling

    1. Institute of Molecular Medicine and Cell Research, University Freiburg, Freiburg, Germany

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1574)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. [14C]-Acetyl-Coenzyme A-Based In Vitro N-Terminal Acetylation Assay

    • Adrian Drazic, Thomas Arnesen
    Pages 1-8

  3. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity

    • Håvard Foyn, Paul R. Thompson, Thomas Arnesen
    Pages 9-15

  4. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation

    • Willy V. Bienvenut, Carmela Giglione, Thierry Meinnel
    Pages 17-34

  5. Profiling of Protein N-Termini and Their Modifications in Complex Samples

    • Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, Pitter F. Huesgen
    Pages 35-50

  6. Protease Substrate Profiling by N-Terminal COFRADIC

    • An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert et al.
    Pages 51-76

  7. Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification

    • Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, Christine Schaeffer-Reiss
    Pages 77-90

  8. Multidimensional Analysis of Protease Substrates and Their Cellular Origins in Mixed Secretomes from Multiple Cell Types

    • Pascal Schlage, Ulrich auf dem Keller
    Pages 91-104

  9. System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates

    • Zon W. Lai, Oliver Schilling
    Pages 105-114

  10. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC

    • Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, Petra Van Damme
    Pages 115-133

  11. ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling

    • Wenwen Duan, Guoqiang Xu
    Pages 135-144

  12. Determining Protease Substrates Within a Complex Protein Background Using the PROtein TOpography and Migration Analysis Platform (PROTOMAP)

    • R. A. Fuhrman-Luck, L. M. Silva, M. L. Hastie, J. J. Gorman, J. A. Clements
    Pages 145-170

  13. Multiplexed Protease Specificity Profiling Using Isobaric Labeling

    • Joanna Tucher, Andreas Tholey
    Pages 171-182

  14. FPPS: Fast Profiling of Protease Specificity

    • Matej Vizovišek, Robert Vidmar, Marko Fonović
    Pages 183-195

  15. Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics

    • Chia-yi Chen, Bettina Mayer, Oliver Schilling
    Pages 197-204

  16. Prediction of Proteases Involved in Peptide Generation

    • Mercedes Arguello Casteleiro, Robert Stevens, Julie Klein
    Pages 205-213

  17. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches

    • Anita Chalasani$, Kyungmin Ji$, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin et al.
    Pages 215-225

  18. Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors

    • Angelina Hahlbrock, Dorothée Gößwein, Roland H. Stauber
    Pages 227-241

  19. Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method

    • Catharina Conrad, Miles A. Miller, Jörg W. Bartsch, Uwe Schlomann, Douglas A. Lauffenburger
    Pages 243-253

  20. Synthesis and Application of Activity-Based Probes for Proteases

    • Tim Van Kersavond, Minh T. N. Nguyen, Steven H. L. Verhelst
    Pages 255-266
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Keywords

About this book

This volume presents detailed protocols for novel strategies and approaches to improve functional understanding of protein N- and C-terminal biology. Protein Terminal Profiling: Methods and Protocols addresses topics such as protease specificity profiling, N-terminal acetylation, assays to probe protease activity in cellular systems, protein N- and C-termini on a proteome-wide scale, and biochemical approaches to explain and examine extracellular protease activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Cutting-edge and thorough, Protein Terminal Profiling: Methods and Protocols is a valuable resource for researchers that focus on biochemistry and cell biology, and those who share a broad interest in protein functionality and protein modifications.

Editors and Affiliations

  • Institute of Molecular Medicine and Cell Research, University Freiburg, Freiburg, Germany

    Oliver Schilling

Bibliographic Information

  • Book Title: Protein Terminal Profiling

  • Book Subtitle: Methods and Protocols

  • Editors: Oliver Schilling

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6850-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6849-7Published: 18 March 2017

  • Softcover ISBN: 978-1-4939-8317-9Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6850-3Published: 17 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 268

  • Number of Illustrations: 16 b/w illustrations, 37 illustrations in colour

  • Topics: Protein Science

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