Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1568)
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Table of contents (27 protocols)
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Front Matter
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Introduction
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Front Matter
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Methodology
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Front Matter
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Advances in Cryotechnology, Research, and Animal Models
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Front Matter
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Keywords
About this book
This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods andProtocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation.
Editors and Affiliations
About the editors
Zsolt Peter Nagy, M.D., Ph.D., is the Scientific Director at Reproductive Biology Associates (RBA), in Atlanta, GA, USA. He has acquired distinctive knowledge and experience on embryo science. He is well known for his contributions to oocyte vitrification and the development of the largest donor oocyte cryo-bank in North America. Dr. Nagy is a member/officer of several national and international professional societies, including ASRM, ESHRE, Alpha, ISFP and AFP, as well as a reviewer and editor. He is the author and/or co-author of over 200 publications, book chapters, and books.
Dr. Alex C. Varghese is the Scientific and Laboratory Director at Astra Fertility group in Mississauga, Canada. His proficiency and vast experience in clinical embryology and academics earned him a reputation in the ART community. He is well regarded for his passion to disseminate knowledge through online platforms and also as a leader of embryology society. He has helped many units establish training programs and affordable batch IVF units in many South Asian Countries. His area of interest is in developing automated systems for assisted reproduction. Dr. Varghese believes in “responsible reproduction” through ethical practices, low-cost IVF for the masses, quality management, and safe practices that safe guard the health of IVF children.
Bibliographic Information
Book Title: Cryopreservation of Mammalian Gametes and Embryos
Book Subtitle: Methods and Protocols
Editors: Zsolt Peter Nagy, Alex C. Varghese, Ashok Agarwal
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6828-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6826-8Published: 26 April 2017
Softcover ISBN: 978-1-4939-8310-0Published: 19 July 2018
eBook ISBN: 978-1-4939-6828-2Published: 18 April 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 372
Number of Illustrations: 26 b/w illustrations, 119 illustrations in colour
Topics: Cell Biology