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Thermogenic Fat

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Jun Wu

    1. Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1566)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Isolation and Culture Methods

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue

      • Wei Gao, Xingxing Kong, Qin Yang
      Pages 3-8

    3. Isolation of Mouse Stromal Vascular Cells for Monolayer Culture

      • Longhua Liu, Louise D. Zheng, Sarah R. Donnelly, Margo P. Emont, Jun Wu, Zhiyong Cheng
      Pages 9-16

    4. Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells

      • Umesh D. Wankhade, Sushil G. Rane
      Pages 17-24

    5. Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes

      • Jochen Steinbring, Antonia Graja, Anne-Marie Jank, Tim J. Schulz
      Pages 25-36

    6. Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells

      • Rohollah Babaei, Irem Bayindir-Buchhalter, Irina Meln, Alexandros Vegiopoulos
      Pages 37-48

    7. Isolation of Immune Cells from Adipose Tissue for Flow Cytometry

      • Jonathan R. Brestoff
      Pages 49-59

    8. Differentiation and Metabolic Interrogation of Human Adipocytes

      • Nicki A. Baker, Lindsey A. Muir, Carey N. Lumeng, Robert W. O’Rourke
      Pages 61-76

    9. Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines

      • Farnaz Shamsi, Yu-Hua Tseng
      Pages 77-85

  3. Functional Analysis, Bioengineering, and Other Applications

    1. Front Matter

      Pages 87-87
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    2. Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue

      • Yun-Hee Lee
      Pages 89-98

    3. Genetic Mouse Models: The Powerful Tools to Study Fat Tissues

      • Xingxing Kong, Kevin W. Williams, Tiemin Liu
      Pages 99-107

    4. Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function

      • Nicolás Gómez-Banoy, James C. Lo
      Pages 109-124

    5. Bioenergetic Analyses in Adipose Tissue

      • Lawrence Kazak
      Pages 125-134

    6. Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry

      • Eun Ran Kim, Qingchun Tong
      Pages 135-143

    7. Isolation and Patch-Clamp of Primary Adipocytes

      • Yanhui Zhang, Dan Tong, Anil Mishra, Litao Xie, Isaac Samuel, Jessica K. Smith et al.
      Pages 145-150

    8. In Vitro Approaches to Model and Study Communication Between Adipose Tissue and the Liver

      • Sean O’Connor, Paul Cohen
      Pages 151-158

    9. Identification and Quantification of Human Brown Adipose Tissue

      • Maria Chondronikola, Craig Porter, John O. Ogunbileje, Labros S. Sidossis
      Pages 159-176

    10. Designing 3-D Adipospheres for Quantitative Metabolic Study

      • Takeshi Akama, Brendan M. Leung, Joe Labuz, Shuichi Takayama, Tae-Hwa Chun
      Pages 177-183
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Keywords

About this book

This volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Writtenin the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Thermogenic Fat: Methods and Protocols is a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions.

Editors and Affiliations

  • Department of Molecular and Integrative Physiology, Life Sciences Institute, University of Michigan, Ann Arbor, USA

    Jun Wu

Bibliographic Information

  • Book Title: Thermogenic Fat

  • Book Subtitle: Methods and Protocols

  • Editors: Jun Wu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6820-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-6819-0Published: 28 February 2017

  • Softcover ISBN: 978-1-4939-8308-7Published: 12 July 2018

  • eBook ISBN: 978-1-4939-6820-6Published: 26 February 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 214

  • Number of Illustrations: 12 b/w illustrations, 30 illustrations in colour

  • Topics: Cell Biology

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