Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1565)
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Table of contents (22 protocols)
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Front Matter
Keywords
About this book
This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinos in utero to developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Morpholino Oligomers: Methods and Protocols aims to provide a diverse application of Morpholinos along with protocols that will assist new labs in moving the frontier.
Editors and Affiliations
Bibliographic Information
Book Title: Morpholino Oligomers
Book Subtitle: Methods and Protocols
Editors: Hong M. Moulton, Jon D. Moulton
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6817-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6815-2Published: 01 April 2017
Softcover ISBN: 978-1-4939-8307-0Published: 20 July 2018
eBook ISBN: 978-1-4939-6817-6Published: 30 March 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 284
Number of Illustrations: 22 b/w illustrations, 38 illustrations in colour
Topics: Biochemistry, general