Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1562)
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Table of contents (20 protocols)
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Front Matter
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Modifications in Abundant RNA Species
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Front Matter
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Analysis of 5-Methylcytosine
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Front Matter
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Further RNA Modifications
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Front Matter
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About this book
This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RNA. The protocols cover topics such as wet-lab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptome-scale data, and protocols for the functional examination of RNA modifications and enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, RNA Methylation: Methods and Protocols is a valuable resource for biochemists and molecular biologists, from various fields, who wish to investigate different types of RNA methylations.
Editors and Affiliations
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Division of Molecular Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria
Alexandra Lusser
Bibliographic Information
Book Title: RNA Methylation
Book Subtitle: Methods and Protocols
Editors: Alexandra Lusser
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6807-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6805-3Published: 28 March 2017
Softcover ISBN: 978-1-4939-8304-9Published: 20 July 2018
eBook ISBN: 978-1-4939-6807-7Published: 27 March 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 304
Number of Illustrations: 28 b/w illustrations, 34 illustrations in colour
Topics: Human Genetics