Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1554)
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Table of contents (21 protocols)
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Front Matter
About this book
This second edition volume provides detailed protocols that address the challenges of signal-transduction IHC. This book delves into chapters that discuss the nature of signal transduction phenomena and approaches to making phosphor-specific antibodies, as well as numerous bona fide methods methods on digital imaging techniques, preservation of tissue targets, multicolor detection, flow cytometry, lipophagy analysis, apoptosis, and the combination of IHC with in situ hybridization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and comprehensive, Signal Transduction Immunohistochemistry: Methods and Protocols, Second Edition is a valuable resource to both novices and experts in other fields of biomedical research who need advice on IHC protocols to study signal transduction. This book will also be useful for researchers in academia, government labs, and the biotech industry.
Editors and Affiliations
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Immunocytochemistry & Elispot Assays, Bio-Techne, Inc., Minneapolis, USA
Alexander E. Kalyuzhny
Bibliographic Information
Book Title: Signal Transduction Immunohistochemistry
Book Subtitle: Methods and Protocols
Editors: Alexander E. Kalyuzhny
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6759-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6757-5Published: 21 February 2017
Softcover ISBN: 978-1-4939-8294-3Published: 20 June 2018
eBook ISBN: 978-1-4939-6759-9Published: 07 February 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: X, 290
Number of Illustrations: 22 b/w illustrations, 45 illustrations in colour
Topics: Immunology