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Adult Stem Cells

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Paolo Di Nardo

    1. Center for Regenerative Medicine, Università di Roma Tor Vergata, Rome, Italy

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  2. Sanjiv Dhingra

    1. Institute of Cardiovascular Sciences St-Boniface Hospital Albrechtsen Research Centre Regenerative Medicine Program College of Medicine Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada

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    You can also search for this editor in PubMed   Google Scholar

  3. Dinender K. Singla

    1. Division of Metabolic and Cardiovascular Sciences Burnett School of Biomedical Sciences, University of Central Florida, Orlando, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1553)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xii
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  2. A Simple Protocol to Isolate, Characterize, and Expand Dental Pulp Stem Cells

    • Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, Giovanni Nicolao Berta
    Pages 1-13

  3. Derivation of Mesenchymal Stem Cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells

    • Glen Lester Sequiera, Niketa Sareen, Ejlal Abu El-Rub, Sanjiv Dhingra
    Pages 15-23

  4. Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren’s-like Disease in NOD Mice

    • Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, Simon D. Tran
    Pages 25-39

  5. Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer

    • Giulia Prosdocimo, Mauro Giacca
    Pages 41-53

  6. Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells

    • Ghazaleh Hajmousa, Martin C. Harmsen
    Pages 55-65

  7. Isolation and In Vitro Characterization of Epidermal Stem Cells

    • Kasper S. Moestrup, Marianne S. Andersen, Kim B. Jensen
    Pages 67-83

  8. Endothelial Progenitor Cells: Procedure for Cell Isolation and Applications

    • Venkata N. S. Garikipati, Raj Kishore
    Pages 85-89

  9. Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID

    • Vikram Sabapathy, Franklin Jebaraj Herbert, Sanjay Kumar
    Pages 91-113

  10. Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID

    • Vikram Sabapathy, Balasubramanian Sundaram, Sanjay Kumar
    Pages 115-132

  11. Isolation, Characterization, and Expansion of Cancer Stem Cells

    • Luke A. Torre-Healy, Artem Berezovsky, Justin D. Lathia
    Pages 133-143

  12. Cardiac Progenitor Cell Extraction from Human Auricles

    • Paolo Di Nardo, Francesca Pagliari
    Pages 145-154

  13. Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle

    • Antonio Musarò, Silvia Carosio
    Pages 155-167

  14. Isolation of Stromal Stem Cells from Adipose Tissue

    • Maria Prat, Francesca Oltolina, Silvia Antonini, Andrea Zamperone
    Pages 169-182

  15. Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells

    • Glen Lester Sequiera, Sekaran Saravanan, Sanjiv Dhingra
    Pages 183-190

  16. A Simplified and Systematic Method to Isolate, Culture, and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth

    • Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi et al.
    Pages 191-207

  17. Measurement of Autophagy by Flow Cytometry

    • Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi, Michele Caraglia
    Pages 209-216

  18. Echocardiography-Guided Intramyocardial Injection Method in a Murine Model

    • Kay Maeda, Rick Seymour, Marc Ruel, Erik J. Suuronen
    Pages 217-225

  19. Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells

    • Federico Franchi, Martin Rodriguez-Porcel
    Pages 227-239

  20. Methods for Long-Term Storage of Murine Bone Marrow-Derived Mesenchymal Stem Cells

    • Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, Sanjiv Dhingra
    Pages 241-248
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Keywords

About this book

This volume contains a collection of protocols from some of the major laboratories involved in stem cell research across the world. The research discussed in this book covers topics such as: isolating, characterizing and expanding dental pulp stem cells; manipulating the proliferative potential of cardiomyocytes by gene transfer; isolation of stromal stem cells from adipose tissue; noninvasive assessment of cell fat and biology in transplanted mesenchymal stem cells; and cell-free therapy for organ repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Adult Stem Cells: Methods and Protocols is a valuable resource for helping researchers transform the study of stem cells into an industrialized process that will supply patients with efficient, safe, and cost-effective cell treatments.

Editors and Affiliations

  • Center for Regenerative Medicine, Università di Roma Tor Vergata, Rome, Italy

    Paolo Di Nardo

  • Institute of Cardiovascular Sciences St-Boniface Hospital Albrechtsen Research Centre Regenerative Medicine Program College of Medicine Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada

    Sanjiv Dhingra

  • Division of Metabolic and Cardiovascular Sciences Burnett School of Biomedical Sciences, University of Central Florida, Orlando, USA

    Dinender K. Singla

Bibliographic Information

  • Book Title: Adult Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Paolo Di Nardo, Sanjiv Dhingra, Dinender K. Singla

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6756-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6754-4Published: 25 February 2017

  • Softcover ISBN: 978-1-4939-8293-6Published: 15 July 2018

  • eBook ISBN: 978-1-4939-6756-8Published: 22 February 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 324

  • Number of Illustrations: 9 b/w illustrations, 54 illustrations in colour

  • Topics: Cell Biology

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