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  • © 2017

Proteomics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keys notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1550)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
  2. A Robust Protocol for Protein Extraction and Digestion

    • Michelle Atallah, Mark R. Flory, Parag Mallick
    Pages 1-10
  3. Profiling Cell Lines Nuclear Sub-proteome

    • Aline Poersch, Andrea G. Maria, Camila S. Palma, Mariana L. Grassi, Daniele Albuquerque, Carolina H. Thomé et al.
    Pages 35-46
  4. Optimized Enrichment of Phosphoproteomes by Fe-IMAC Column Chromatography

    • Benjamin Ruprecht, Heiner Koch, Petra Domasinska, Martin Frejno, Bernhard Kuster, Simone Lemeer
    Pages 47-60
  5. Hydrophilic Strong Anion Exchange (hSAX) Chromatography Enables Deep Fractionation of Tissue Proteomes

    • Benjamin Ruprecht, Dongxue Wang, Riccardo Zenezini Chiozzi, Li-Hua Li, Hannes Hahne, Bernhard Kuster
    Pages 69-82
  6. Parallel Exploration of Interaction Space by BioID and Affinity Purification Coupled to Mass Spectrometry

    • Geoffrey G. Hesketh, Ji-Young Youn, Payman Samavarchi-Tehrani, Brian Raught, Anne-Claude Gingras
    Pages 115-136
  7. LUMIER: A Discovery Tool for Mammalian Protein Interaction Networks

    • Miriam Barrios-Rodiles, Jonathan D. Ellis, Benjamin J. Blencowe, Jeffrey L. Wrana
    Pages 137-148
  8. Dual-Color, Multiplex Analysis of Protein Microarrays for Precision Medicine

    • Solomon Yeon, Florian Bell, Michael Shultz, Grace Lawrence, Michael Harpole, Virginia Espina
    Pages 149-170
  9. Quantitative Proteomics Using SILAC

    • Kian Kani
    Pages 171-184
  10. Pathway-Informed Discovery and Targeted Proteomic Workflows Using Mass Spectrometry

    • Caroline S. Chu, Christine A. Miller, Andy Gieschen, Steve M. Fischer
    Pages 199-221
  11. Protein Micropatterning Assay: Quantitative Analysis of Protein–Protein Interactions

    • Gerhard J. Schütz, Julian Weghuber, Peter Lanzerstorfer, Eva Sevcsik
    Pages 261-270
  12. Designing Successful Proteomics Experiments

    • Daniel Ruderman
    Pages 271-288

About this book

This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Keck School of Medicine, University of Southern California, Los Angeles, USA

    Lucio Comai, Jonathan E. Katz

  • Stanford University, Palo Alto, USA

    Parag Mallick

Bibliographic Information

  • Book Title: Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Lucio Comai, Jonathan E. Katz, Parag Mallick

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6747-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-6745-2Published: 11 February 2017

  • Softcover ISBN: 978-1-4939-8290-5Published: 09 June 2018

  • eBook ISBN: 978-1-4939-6747-6Published: 10 February 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 371

  • Number of Illustrations: 16 b/w illustrations, 48 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access