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Microchip Diagnostics

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Valérie Taly

    1. INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, Equipe labellisée Ligue Nationale contre le cancer 2016, Paris, France

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  2. Jean-Louis Viovy

    1. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

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  3. Stéphanie Descroix

    1. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1547)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Part I

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Microfluidics-Enabled Diagnostic Systems: Markets, Challenges, and Examples

      • Holger Becker, Claudia Gärtner
      Pages 3-21

  3. Microchips for Protein Bioassays

    1. Front Matter

      Pages 23-23
      Download chapter PDF

    2. Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Efficient Fabrication and Sealing of Chips Using a “Chip-Olate” Process

      • Yuksel Temiz, Emmanuel Delamarche
      Pages 25-36

    3. Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Patterning Capture Antibodies Using Microcontact Printing and Dry-Film Resists

      • Yuksel Temiz, Robert D. Lovchik, Emmanuel Delamarche
      Pages 37-47

    4. Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars

      • Toshihiro Kasama, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
      Pages 49-56

    5. Using Electrophoretic Immunoassay to Monitor Hormone Secretion

      • Shusheng Lu, Robert T Kennedy
      Pages 57-67

    6. On-Chip Magnetic Particle-Based Immunoassays Using Multilaminar Flow for Clinical Diagnostics

      • Mark D. Tarn, Nicole Pamme
      Pages 69-83

    7. Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in Femtoliter-Sized Reaction Wells for Single-Molecule Detection

      • Deborah Decrop, Elena Pérez Ruiz, Phalguni Tewari Kumar, Lisa Tripodi, Tadej Kokalj, Jeroen Lammertyn
      Pages 85-101

  4. Microchips for Nucleic Acids Analysis

    1. Front Matter

      Pages 103-103
      Download chapter PDF

    2. Microfluidic DNA Stretching Device for Single-Molecule Diagnostics

      • Daisuke Onoshima, Yoshinobu Baba
      Pages 105-111

    3. Droplet Microfluidic and Magnetic Particles Platform for Cancer Typing

      • Davide Ferraro, Jérôme Champ, Bruno Teste, M. Serra, Laurent Malaquin, Stéphanie Descroix et al.
      Pages 113-121

    4. SlipChip Device for Digital Nucleic Acid Amplification

      • Feng Shen
      Pages 123-132

    5. Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion

      • Deniz Pekin, Valerie Taly
      Pages 133-142

    6. Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations

      • Deniz Pekin, Valerie Taly
      Pages 143-164

  5. Microfluidic Chips for Cell-Based Enrichment and Analysis

    1. Front Matter

      Pages 165-165
      Download chapter PDF

    2. Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic Chip

      • Hamizah A. Cognart, Chia-Pin Chang
      Pages 167-174

    3. Microfluidic-Based Bacteria Isolation from Whole Blood for Diagnostics of Blood Stream Infection

      • Sergey Zelenin, Harisha Ramachandraiah, Asim Faridi, Aman Russom
      Pages 175-186

    4. Microfluidic Platform for Parallel Single Cell Analysis for Diagnostic Applications

      • Séverine Le Gac
      Pages 187-209
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Keywords

About this book

This volume illustrates how microfluidic approaches can meet the requirement of clinical diagnosis based on molecular or cellular biomarkers. Microchip Diagnostics: Methods and Protocols is divided into four sections describing the business aspects of the microfluidic-based systems for diagnosis, demonstrating how versatile microfluidics can be regarding to protein bioassay integration, presenting microfluidic approaches for nucleic analysis based on mono or diphasic format, and highlighting recent contributions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Editors and Affiliations

  • INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, Equipe labellisée Ligue Nationale contre le cancer 2016, Paris, France

    Valérie Taly

  • Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Jean-Louis Viovy, Stéphanie Descroix

Bibliographic Information

  • Book Title: Microchip Diagnostics

  • Book Subtitle: Methods and Protocols

  • Editors: Valérie Taly, Jean-Louis Viovy, Stéphanie Descroix

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6734-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6732-2Published: 03 January 2017

  • Softcover ISBN: 978-1-4939-8286-8Published: 08 June 2018

  • eBook ISBN: 978-1-4939-6734-6Published: 02 January 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 223

  • Number of Illustrations: 10 b/w illustrations, 74 illustrations in colour

  • Topics: Biotechnology

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