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  • Book
  • © 2017

Synapse Development

Methods and Protocols

Editors:

  1. Alexandros Poulopoulos

    1. Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, USA

    View editor publications

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  • Includes cutting-edge methods and protocols for the study of synapse development
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1538)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    PDF

  2. Forming Synapses in a Dish

    1. Front Matter

      Pages 1-1
      PDF

    4. Synaptogenic Assays Using Neurons Cultured on Micropatterned Substrates

      • Katalin Czöndör, Olivier Thoumine
      Pages 29-44

    6. Generation of Spinal Motor Neurons from Human Pluripotent Stem Cells

      • David P. Santos, Evangelos Kiskinis
      Pages 53-66

  3. Analyzing Synaptic Constituents

    1. Front Matter

      Pages 67-67
      PDF

    2. Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins

      • Bekir Altas, Olaf Jahn, Hiroshi Kawabe
      Pages 69-82

    3. In Situ Protein Binding Assay Using Fc-Fusion Proteins

      • Nirmala Padmanabhan, Tabrez J. Siddiqui
      Pages 83-91

    5. Isolation of Synaptosomes, Synaptic Plasma Membranes, and Synaptic Junctional Complexes

      • Mary L. Michaelis, Lei Jiang, Elias K. Michaelis
      Pages 107-119

    6. Purification of Synaptosome Populations Using Fluorescence-Activated Synaptosome Sorting

      • Elisa Luquet, Christoph Biesemann, Annie Munier, Etienne Herzog
      Pages 121-134

  4. Observing Synaptic Structure and Ultrastructure

    1. Front Matter

      Pages 135-135
      PDF

    2. Optimized Protocol for Imaging Cleared Neural Tissues Using Light Microscopy

      • Yoh Isogai, Douglas S. Richardson, Catherine Dulac, Joseph Bergan
      Pages 137-153

    3. Structured Illumination Microscopy for the Investigation of Synaptic Structure and Function

      • Soyon Hong, Daniel K. Wilton, Beth Stevens, Douglas S. Richardson
      Pages 155-167

    4. 3D d STORM Imaging of Fixed Brain Tissue

      • Frank Herrmannsdörfer, Benjamin Flottmann, Siddarth Nanguneri, Varun Venkataramani, Heinz Horstmann, Thomas Kuner et al.
      Pages 169-184

  5. Following Synapse Dynamics

    1. Front Matter

      Pages 233-233
      PDF
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About this book

This detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures, their dynamics, and the assemblies they form, all in the structure of a laboratory guide. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and  avoiding known pitfalls. 

Authoritative and practical, Synapse Development: Methods and Protocols serves as an ideal guide to minimizing the barrier to entry for the integration of new approaches with existing expertise, producing syntheses that will foster novel perspectives on the many ways in which synapses form, transform, and transmit.
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Keywords

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Editors and Affiliations

  • Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, USA

    Alexandros Poulopoulos

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Bibliographic Information

  • Book Title: Synapse Development

  • Book Subtitle: Methods and Protocols

  • Editors: Alexandros Poulopoulos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6688-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6686-8Published: 10 December 2016

  • Softcover ISBN: 978-1-4939-8273-8Published: 06 July 2018

  • eBook ISBN: 978-1-4939-6688-2Published: 10 December 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 372

  • Number of Illustrations: 12 b/w illustrations, 67 illustrations in colour

  • Topics: Neurosciences

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Buy it now

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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