Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains keynotes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1535)
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Table of contents (23 protocols)
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Front Matter
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Identification and Characterization of Bacterial Effector Molecules
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Front Matter
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Structural Biology of Bacterial–Host Interactions
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Front Matter
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Genetics and Phylogenetics of Bacterial Pathogens
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Front Matter
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Bacterial Imaging Approaches and Related Techniques
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Front Matter
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About this book
This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as Streptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumonia, Listeria monocytogenes, and Mycobacterium marinum. Bacterial Pathogens: Methods and Protocols is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Bacterial Pathogens: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.
Editors and Affiliations
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Department of Clinical Sciences, Lund University, Division of Infection Medicine, Lund, Sweden
Pontus Nordenfelt, Mattias Collin
Bibliographic Information
Book Title: Bacterial Pathogenesis
Book Subtitle: Methods and Protocols
Editors: Pontus Nordenfelt, Mattias Collin
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6673-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6671-4Published: 02 December 2016
Softcover ISBN: 978-1-4939-8268-4Published: 07 July 2018
eBook ISBN: 978-1-4939-6673-8Published: 01 December 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 357
Number of Illustrations: 23 b/w illustrations, 48 illustrations in colour
Topics: Microbiology