Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1523)
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Table of contents (28 protocols)
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Front Matter
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Native Tau Protein: Conformational Studies and Tubulin Interactions
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Front Matter
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In Vitro Self-Association of Tau Protein: Oligomer and Fibril Formation
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Front Matter
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Tau Post-Translational Modifications
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Front Matter
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Detecting Tau Proteins, Their Modifications, Mutations and Interacting Partners
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Front Matter
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Keywords
About this book
This detailed volume gathers basic and advanced methods and protocols from in vitro assays and in vivo models to address the molecular and functional aspects of tau physiopathology. Divided into five parts that illustrate the underlying molecular mechanisms of Tau functions and dysfunctions in Alzheimer’s disease and other dementia referred to as tauopathies, this volume discusses timely topics such as: conformational studies of native tau protein and investigation of its physiological function in microtubules binding and tubulin polymerization; in vitro methods of formation and detection of tau oligomers and PHF-like fibrils; characterization and in vitro introduction of post-translational modifications on tau proteins for further functional studies; analytical tools for the detection of tau proteins, their modifications and cellular interactions, and MAPT gene mutations in various biological samples; and cellular and in vivo models for the investigations of tau physiopathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Tau Protein: Methods and Protocols is a valuable resource that addresses the most recent aspects of Alzheimer’s disease research related to Tau protein and state-of-the-art experimental techniques. It targets a broad scientific audience including molecular biologists and biochemists, as well as all researchers interested in exploring the functions of tau proteins.
Editors and Affiliations
Bibliographic Information
Book Title: Tau Protein
Book Subtitle: Methods and Protocols
Editors: Caroline Smet-Nocca
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6598-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6596-0Published: 14 December 2016
Softcover ISBN: 978-1-4939-8246-2Published: 07 July 2018
eBook ISBN: 978-1-4939-6598-4Published: 12 December 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVIII, 432
Number of Illustrations: 19 b/w illustrations, 64 illustrations in colour
Topics: Protein Science