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Cardiac Gene Therapy

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Kiyotake Ishikawa

    1. Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, USA

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  • Includes cutting-edge methods and protocols for the study of cardiac gene therapy
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1521)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Current Methods in Cardiac Gene Therapy: Overview

      • Kiyotake Ishikawa, Roger J. Hajjar
      Pages 3-14

  3. Genetic Modification

    1. Front Matter

      Pages 15-15
      Download chapter PDF

    2. Silencing Genes in the Heart

      • Henry Fechner, Roland Vetter, Jens Kurreck, Wolfgang Poller
      Pages 17-39

    3. Generation of Efficient miRNA Inhibitors Using Tough Decoy Constructs

      • Jimeen Yoo, Roger J. Hajjar, Dongtak Jeong
      Pages 41-53

    4. Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro

      • Vittavat Termglinchan, Timon Seeger, Caressa Chen, Joseph C. Wu, Ioannis Karakikes
      Pages 55-68

    5. Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarction

      • Hong Ma, Li Wang, Jiandong Liu, Li Qian
      Pages 69-88

  4. Gene Delivery Vectors

    1. Front Matter

      Pages 89-89
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    2. Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9

      • Erik Kohlbrenner, Thomas Weber
      Pages 91-107

    3. Cell-Based Measurement of Neutralizing Antibodies Against Adeno-Associated Virus (AAV)

      • Andreas Jungmann, Oliver Müller, Kleopatra Rapti
      Pages 109-126

    4. Synthesis of Modified mRNA for Myocardial Delivery

      • Jason Kondrat, Nishat Sultana, Lior Zangi
      Pages 127-138

    5. Exosomes-Based Gene Therapy for MicroRNA Delivery

      • Prabhu Mathiyalagan, Susmita Sahoo
      Pages 139-152

    6. Lipidoid mRNA Nanoparticles for Myocardial Delivery in Rodents

      • Irene C. Turnbull, Ahmed A. Eltoukhy, Daniel G. Anderson, Kevin D. Costa
      Pages 153-166

  5. Gene Delivery Methods

    1. Front Matter

      Pages 167-167
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    2. Gene Transfer in Isolated Adult Cardiomyocytes

      • Kjetil Hodne, David B. Lipsett, William E. Louch
      Pages 169-182

    3. Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells

      • Francesca Stillitano, Ioannis Karakikes, Roger J. Hajjar
      Pages 183-193

    4. Gene Transfer to Rodent Hearts In Vivo

      • Federica del Monte, Kiyotake Ishikawa, Roger J. Hajjar
      Pages 195-204

    5. Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery

      • Shuyuan Chen, Paul A. Grayburn
      Pages 205-218

    6. A Needleless Liquid Jet Injection Delivery Approach for Cardiac Gene Therapy

      • Anthony S. Fargnoli, Michael G. Katz, Charles R. Bridges
      Pages 219-226
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Keywords

About this book

This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients’ outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field.

Editors and Affiliations

  • Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, USA

    Kiyotake Ishikawa

Bibliographic Information

  • Book Title: Cardiac Gene Therapy

  • Book Subtitle: Methods and Protocols

  • Editors: Kiyotake Ishikawa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6588-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6586-1Published: 02 December 2016

  • Softcover ISBN: 978-1-4939-8243-1Published: 06 July 2018

  • eBook ISBN: 978-1-4939-6588-5Published: 30 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 355

  • Number of Illustrations: 15 b/w illustrations, 78 illustrations in colour

  • Topics: Molecular Medicine

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