Human Fungal Pathogen Identification

1. Front Matter

   Pages i-xi

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2. Introductory Chapters

   1. Front Matter

      Pages 1-1

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   2. Current Challenges in the Diagnosis of Fungal Infections

      • Cornelia Lass-Flörl

      Pages 3-15

   3. The Changing Epidemiology of Invasive Fungal Infections

      • David A. Enoch, Huina Yang, Sani H. Aliyu, Christianne Micallef

      Pages 17-65

   4. Current Algorithms in Fungal Diagnosis in the Immunocompromised Host

      • Thomas Lehrnbecher, Karsten Becker, Andreas H. Groll

      Pages 67-84

   5. Commercial Molecular Tests for Fungal Diagnosis from a Practical Point of View

      • Michaela Lackner, Cornelia Lass-Flörl

      Pages 85-105

   6. Systemic Antifungal Agents: Current Status and Projected Future Developments

      • Seyedmojtaba Seyedmousavi, Haleh Rafati, Macit Ilkit, Ali Tolooe, Mohammad T. Hedayati, Paul Verweij

      Pages 107-139

   7. Fungal-Grade Reagents and Materials for Molecular Analysis

      • Michael G. Lorenz, Michael Lustig, Marina Linow

      Pages 141-150

3. Host Susceptibility and Defense

   1. Front Matter

      Pages 151-151

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   2. Host-Derived Biomarkers for Risk Assessment of Invasive Fungal Diseases

      • Cristina Cunha, Samuel M. Gonçalves, Agostinho Carvalho

      Pages 153-165

   3. Assessment of Immune Responses to Fungal Infections: Identification and Characterization of Immune Cells in the Infected Tissue

      • Florian Sparber, Salomé LeibundGut-Landmann

      Pages 167-182

4. Screening Approaches to Fungal Pathogen Detection

   1. Front Matter

      Pages 183-183

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   2. Histopathology

      • Renate Kain

      Pages 185-193

   3. Culture-Based Techniques

      • Birgit Willinger

      Pages 195-207

   4. Serological Approaches

      • Barbora Weinbergerova, Iva Kocmanova, Zdenek Racil, Jiri Mayer

      Pages 209-221

   5. Isolation of Nucleic Acids for Fungal Diagnosis

      • P. Lewis White, Rosemary A. Barnes

      Pages 223-247

   6. Prerequisites for Control of Contamination in Fungal Diagnosis

      • Stefan Czurda, Thomas Lion

      Pages 249-255

   7. Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques

      • Stefan Czurda, Thomas Lion

      Pages 257-266

   8. Genus- and Species-Specific PCR Detection Methods

      • Jan Springer, Jürgen Löffler

      Pages 267-279

   9. Identification of Fungal Pathogens in Tissue Samples from Patients with Proven Invasive Infection by Fluorescence In Situ Hybridization

      • Ilka McCormick Smith, Volker Rickerts

      Pages 281-288

This detailed volume presents timely and authoritative content offering a comprehensive overview of the current state of the art in fungal diagnostics. Moreover, it addresses on-going developments expected to provide a basis for targeted treatment strategies resulting in improved outcome of invasive mycoses. The knowledge of host-related predisposing factors and stratified treatment options facilitating timely onset of adequate antifungal therapy are critical for successful clinical management and outcome of invasive fungal disease (IFD), requiring not only rapid diagnosis of a fungal infection and identification of the causative species, but also assessment of pathogen/host factors related to pathogenicity, susceptibility, and response to treatment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tipson troubleshooting and avoiding known pitfalls.

Authoritative and practical, Human Fungal Pathogen Identification: Methods and Protocols serves as an ideal reference for researchers investigating the ever-growing worldwide healthcare problems involving fungal infections.

• Invasive fungal disease (IFD)
• Fungal infections
• Antifungal therapy
• Pathogenicity prediction
• Clinical drug resistance
• Invasive mycoses

• Children’s Cancer Research Institute (CCRI), St.Anna Kinderkrebsforschung, Vienna, Austria

  Thomas Lion

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