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  • © 2017

Hepatocyte Transplantation

Methods and Protocols

  • Includes cutting-edge method and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1506)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. Alternative Cell Sources to Adult Hepatocytes for Hepatic Cell Therapy

      • Eugenia Pareja, María José Gómez-Lechón, Laia Tolosa
      Pages 17-42
  3. Experimental Hepatocyte Transplantation

    1. Front Matter

      Pages 43-43
    2. Successful Engraftment of Human Hepatocytes in uPA-SCID and FRG® KO Mice

      • Lander Foquet, Elizabeth M. Wilson, Lieven Verhoye, Markus Grompe, Geert Leroux-Roels, John Bial et al.
      Pages 117-130
    3. Gunn Rats as a Surrogate Model for Evaluation of Hepatocyte Transplantation-Based Therapies of Crigler–Najjar Syndrome Type 1

      • Zsuzsanna Polgar, Yanfeng Li, Xia Li Wang, Chandan Guha, Namita Roy-Chowdhury, Jayanta Roy-Chowdhury
      Pages 131-147
    4. Experimental Hepatocyte Transplantation in Pigs

      • Eiji Kobayashi, Shin Enosawa, Hiroshi Nagashima
      Pages 149-160
    5. Preclinical Swine Models for Monitoring of Hepatocyte Transplantation by MRI

      • Nathanael Raschzok, Ulf Teichgräber, Johann Pratschke, Igor M. Sauer
      Pages 201-214
  4. Clinical Hepatocyte Transplantation

    1. Front Matter

      Pages 229-229

About this book

This volume features up-to-date protocols for the isolation, preservation, and validation of various cell sources comprising large and small animal models, examining the impact of cell transplantation on acute and chronic liver diseases. Hepatocyte Transplantation: Methods and Protocols guides readers through laboratory protocols for the generation of humanized livers for the assessment of biological actions in vivo and techniques to monitor cell engraftment after cell transplantation in vivo are described and procedures for computational analyses of hepatocyte transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Hepatocyte Transplantation: Methods and Protocols is an essential resource for researchers and clinicians to assess the biological as well as the therapeutic potential of hepatocyte transplantation.


Editors and Affiliations

  • Department of Surgery, University of Leipzig, Leipzig, Germany

    Peggy Stock, Bruno Christ

Bibliographic Information

  • Book Title: Hepatocyte Transplantation

  • Book Subtitle: Methods and Protocols

  • Editors: Peggy Stock, Bruno Christ

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6506-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6504-5Published: 10 November 2016

  • Softcover ISBN: 978-1-4939-8221-9Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6506-9Published: 09 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 365

  • Number of Illustrations: 16 b/w illustrations, 52 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access